Consumables

rAAV-CAG-DIO-FLP-ERT2-WPRE-hGH polyAVirus construct--
pAAV.hSynapsin.SF-iGluSnFR.A184SVirus constructPI: Looger. Activity: Constitutive-
rAAV-nEf1α-FDIO-EYFP-WPRE-hGH polyAVirus construct--
rAAV-TRE3g-EYFP-P2A-CRE-WPRE-hGH polyAVirus construct--
mDlx-HBB-chI-dlox-TVA_2A_EGFP_2A_oG(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV-CaMKIIa-C1V1(E122T/E162T)-TS-EYFP Virus constructThird-generation Optogenetic Inhibition: eNpHR3.0-
AAV9-EFS-DIO-OXT1.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
rAAV-hSyn-NES-His-CaMPARI2-L398T-Flag-HA-Myc-WPRE-SV40 polyAVirus construct--
rAAV-hSyn-CRE-WPRE-hGH polyAVirus construct--
rAAV-CAG-dLight1.1-WPRE-hGH polyAVirus construct--
rAAV-mOXT-hM3D(Gq)-mCherry-WPRE-hGH polyAVirus construct--
TRE-DIO-eYFPVirus constructExpression System: Cre-dependent and Tetracycline-inducible. Activity: Cre-dependent, Tet-inducible expression of EYFP. PI: Gradinaru-
rAAV-L7-hChR2(E123T/T159C)-Mcherry-WPRE-hGH polyAVirus construct--
pAAV-EF1a-FlpoVirus constructPI: Deisseroth-
rAAV-EF1α-DIO-NES-jRGECO1a-WPRE-hGH polyAVirus construct--
rAAV-Dlx5/6-mCherry-WPRE-hGH polyAVirus construct--
pAAV-ihSyn1-tTAVirus constructExpression System: Inducible Synapsin promoter (ihSyn). Activity: Expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified tTA expression.. PI: Gradinaru-
hEF1α-dFRT-ArchT_EGFP(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-hSyn-Kir2.1-P2A-EGFP-WPRE-hGH polyAVirus construct--
rAAV-GFAP-CRE-WPRE-hGH polyAVirus construct--
AAV9-hsyn-5HT2LVirus constructTiter: ≥1*10E13vg/ml, 50ul-
pAAV.CamKII(1.3).eYFP.WPRE.hGHVirus constructPI: Deisseroth. Activity: Constitutive-
shortCAG-hChR2(H134R)_dTomato(non-c.d.)-WPRE-SV40p(A)Virus constructsingle-stranded genome-
AAV9-hSyn-eArch3.0-EYFPVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
Other virus constructVirus construct-1
pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGHVirus constructActivity: Constitutive. PI: Deisseroth-
hGFAP-hHBbI/E-cdATeam1.03-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-hSyn-dLight1.1-WPRE-hGH polyAVirus construct--
lenti MS2-P65-HSF1_HygroVirus constructMS2-P65-HSF1 activator helper. Hygromycin resistance. EF1a promoter. PI: Zhang.-
rAAV-Dlx-hChR2(H134R)-mCherry-WPRE-hGH polyAVirus construct--
rAAV-EFS-DIO-CRF3.0(1)-WPRE-hGH polyAVirus construct--
AAV-CaMKIIa-OptoD1-EYFPVirus constructOpto-XRs  (AAV5)-
AAV9-hsyn-DIO-VIP1.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
rAAV-TBG-Luciferase-WPRE-hGH polyAVirus construct--
mCry1-dlox-chI(rev)-mCry1I(rev)-Venus_NLS_Pest(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV-nEF-NpHR3.3-EYFPVirus constructActivity: Constitutive. PI: Deisseroth-
rAAV-CaMKIIa-hM3D(Gq)-EGFP-WPRE-hGH polyAVirus construct--
rAAV-CaMKIIa-ReachR-citrine-WPRE-hGH polyAVirus construct--
hSyn1-chI[4x:sh(NS)]-EGFP-WPRE-SV40p(A)Virus constructsingle-stranded genome-
AAV-CAG-GFPVirus constructPI: Boyden. Activity: Constitutive-
pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPREVirus constructActivity: Constitutive. PI: Deisseroth-
rAAV-TRE3G-DIO-BFP-2A-TVA-2A-RVG-WPREsVirus construct--
pAAV.TBG.PI.eGFP.WPRE.bGHVirus constructPI: Wilson. Activity: Constitutive-
AAV-hSyn-Con/Foff hChR2(H134R)-EYFP-WPREVirus constructINTRSECT (AAV5 & DJ)-
shortCAG-mLCK (N-term.)_mCerulean3-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hEF1α/hTLV1-Con/Don(HA_hM3D(Gq))-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV-CaMKIIa-mScarletVirus constructPI: Deisseroth. Activity: Constitutive-
shortCAG-EGFP-SV40p(A)Virus constructself-complementary genome-
hSyn1-chI-dlox-MitoTimer(rev)-dlox-SV40p(A)Virus constructself-complementary genome-
AAV9-GfaABC1D-GCaMP6fVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
rAAV-Ef1α-DIO-EGFP-2A-TVA -WPRE-hGH polyAVirus construct--
shortCAG-dlox-Jaws_KGC_tdTomato_ER2(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-lox71-Lifeact_jGCaMP8m_mCyRFP1(rev)-lox66-WPRE-hGHp(A)Virus constructsingle-stranded genome-
shortCAG-dlox-hChR2(H134R)_EYFP-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-GfaABC1D-ATP1.0-GAP43-jRGECO1a-SV40 polyAVirus construct--
hEF1α-dlox-SwiChRca_MTS_EYFP(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-CaMKIIa-Jaws-KGC-EGFP-ER2-WPRE-SV40 polyAVirus construct--
AAV-DJ-CAG-mCherryVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
hGFAP-HA_hM4D(Gi)_IRES_mCitrine-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-CX3CR1-EGFP-WPRE-hGH polyAVirus construct--
RG-pLV-VSVG-KanVirus constructFormat: Plasmid DNA, E. Coli Selection: Kan, Total Length: 6456 bp-
rAAV-cfos-TTA(H100Y)-NLS-Flag-WPRE-bGH polyAVirus construct--
rAAV-hSyn-ATP1.0Virus construct--
hSyn1-eNpHR3.0_EYFP-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-GFAP-hM3D(Gq)-mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
rAAV-cfos-TTA-TRE3g-mCherry-WPRE-bGH polyAVirus construct--
rAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPRE-hGH polyAVirus construct--
pTight-hChR2(H134R)_EYFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV7-CAG-mCherryVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
AAV-CAG-FLEX-GFPVirus constructControl Vectors-
AAV-CaMKII-hChR2(T159C)-p2A-EYFP-WPREVirus constructCell Filling Variants-
Human gRNA pooled library in lentiCRISPRv2Virus constructHuman sgRNA library with SpCas9. 76,441 sgRNAs targeting 19,114 genes. Puromycin selection. PI: Doench, Root.-
rAAV-hSyn-CRE-P2A-EGFP-WPREsVirus construct--
rAAV-mOXTR-DIO-mCherry-WPRE-hGH polyAVirus construct--
hSyn1-dlox-GCaMP6m_mRpL10a(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-GfaABC1D-cATP1.0(chick)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV-CAG-hChR2-H134R-tdTomatoVirus constructActivity: Constitutive. *. PI: Svoboda-
AAV9-hSyn-cATP1.0-TESVirus constructTiter: ≥1*10E13vg/ml,50ul-
AAV pCAG-FLEX-EGFP-WPREVirus constructPI: Zeng. Activity: Cre dependent-
rAAV-Dlx5/6-DIO-EYFP-WPRE-hGH polyAVirus construct--
rAAV-TH-NLS-CRE-WPRE-hGH polyAVirus construct--
rAAV-nEf1α-FDIO-RVG-WPRE-hGH polyAVirus construct--
AAV9-hsyn-DIO-NE2hVirus constructTiter: ≥1*10E13vg/ml, 50ul-
hSyn1-lox71-ePdCO_mScarlet(rev)-lox66-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AiE0780m-HBB-chI-iCre-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-EF1α-DIO-hM4D(Gi)-EYFP-WPREsVirus construct--
rAAV-hSyn-Chronos-EGFP-WPRE-bGH polyAVirus construct--
shortCAG-dlox-NES_jRCaMP1b(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hSyn1-dlox-mCherry(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-Jaws_KGC_tdTomato_ER2-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-CaMKIIa-hChR2(H134R)-EYFP-WPRE-hGH polyAVirus construct--
mCaMKIIα(short)-eOPN3_mScarlet-WPRE-synp(A)Virus constructsingle-stranded genome-
AAV5-GfaABC1D-Ado1.0m-mRuby3Virus constructTiter: ≥1*10E13vg/ml,50ul-
AAV9-hSyn-GCaMP6m-P2A-nls-dTomatoVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
rAAV-D2-CRE-EGFP-WPRE-bGH polyAVirus construct--
pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3Virus constructActivity: Cre dependent. PI: Adesnik-
hCMV-chI-Lck_EGFP-SV40p(A)Virus constructself-complementary genome-
AAV9-hSyn-tdTomatoVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
rAAV-hSyn-NES-His-CaMPARI2-Flag-HA-Myc-WPRE-SV40 polyAVirus construct--
rAAV-CaMKIIa-DIO-ChETA(E123A R134H)-EYFP-WPRE-hGH polyAVirus construct--

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

  • Optic fiber designs: Thin, flexible fibers used for transmitting light signals, crucial for optical neural imaging and optogenetic manipulation of neurons.
  • Silicon probe designs: Microfabricated devices designed for recording or stimulating neural activity, allowing for high-resolution interfacing with neural tissue.
  • Virus constructs: Genetically engineered viruses used for delivering genes into neurons, enabling the study of gene function or the manipulation of neural activity.
  • Wire electrodes: Thin metal wires used for recording electrical signals from or delivering electrical stimulation to neurons, essential for electrophysiological studies.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.