Consumables

AAV9-sparse-5-HT1.0(5-HT2.1)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
pAAV-CAG-tTAVirus constructExpression System: CAG-driven, constitutive. Activity: Expression of the tet-off transactivator (tTA). PI: Gradinaru-
AAV2 retro-CMV-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
AAV9-hsyn-CCK2.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
rAAV-CMV-DIO-EGFP-2A-TK-WPRE-hGH polyAVirus construct--
hSyn1-jGCaMP8s_mRpL10a-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-TRE-fDIO-eCB2.0-IRES-tTAVirus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV-hSyn-Flex-Jaws-KGC-GFP-ER2(AAV8 only currently)Virus constructNeural Silencers - Jaws (Halo57) (no AAV6)-
SMF-28-J9Optic fiber designSingle Mode Fiber, 1260 - 1625 nm, Ø900 µm Hytrel Jacket-
hEF1α-YCNano140-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-GFAP-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
AAV-CAG-jGCaMP8m-WPREVirus constructPI: Looger. Activity: Constitutive-
pZac2.1-GfaABC1D-lck-jGCaMP8mVirus constructPI: Looger. Activity: Constitutive-
rAAV-hSyn-DIO-His-EGFP-2A-TVA -WPRE-hGH polyAVirus construct--
hSyn1-chI-mTagBFP2_2A_mSyp1_tdTomato-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-NLS_EGFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-hSyn-mGFP-T2A-Synaptophysin-mRuby-WPRE-hGH polyAVirus construct--
pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3Virus constructPI: GENIE. Activity: Constitutive-
{in progress}Virus constructsingle-stranded genome-
rAAV-Ef1α-DIO-EGFP-2A-cmTK-WPRE-hGH polyAVirus construct--
S630-HPOptic fiber designSingle Mode Fiber, 630 - 860 nm, Ø125 µm Cladding-
rAAV-EFS-DIO-OXT1.0-WPRE-hGH polyAVirus construct--
rAAV- GfaABC1D-cyto-GCaMP6f-SV40 polyAVirus construct--
AAV2 retro-hSyn-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
mCaMKIIα-ChrimsonR_mCherry-WPRE-hGHp(A)Virus constructsingle-stranded genome-
A8x1-tet-2mm-200-121Silicon probe design*This probe has an non-uniform layout. See company catalog for more information: 2018ProbeCatalog_20180515_Web, p.52 Another site with an area of 4200µm^2 is located on the fourth shank from the left, 500µm above the tetrode-
Morris CellTag Barcode Library Version 3Virus construct5,737 barcodes for single-cell clonal analysis. Expresses EGFP. PI: Morris.-
lentiCas9-BlastVirus constructExpresses S. pyogenes Cas9. Blasticidin resistance. EFS promoter. PI: Zhang.-
pAAV-Ef1a-fDIO-ChrimsonR-tdTomatoVirus constructActivity: Flp dependent. PI: Jensen-
AAV9-TRE3G-mCherry-WPRE-PAVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
rAAV-EF1α-DIO-FLP-plus-WPRE-hGH polyAVirus construct--
rAAV-RK-EGFP-WPRE-hGH polyAVirus construct--
AAV9-hSyn-CRF3.0-mScarletVirus constructTiter: ≥1*10E13vg/ml, 50ul-
rAAV-Hypocretin-CRE-WPRE-hGH polyAVirus construct--
rAAV-CaMKIIa -DIO-hM3D(Gq)-mCherry-WPRE-hGH polyAVirus construct--
rAAV-hSyn-dLight1.2-WPRE-pAVirus construct--
AAV9-hSyn-GCaMP6sVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
hGFAP-HA_hM3D(Gq)_IRES_mCitrine-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-dlox-RCaMP1.07(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-VGAT1-CRE-WPRE-hGH polyAVirus construct--
mCaMKIIα(short)-(pre)eGRASP(p30; green)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-dlox-mCherry_2A_FLPo(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Cre dependent-
pENN.AAV.CB7.CI.eGFP.WPRE.rBGVirus constructPI: Wilson. Activity: Constitutive-
pAAV-Ef1a-oScarletVirus constructPI: Deisseroth. Activity: Constitutive-
pAAV-mDlx-ChR2-mCherry-Fishell-3Virus constructActivity: Constitutive. PI: Fishell-
hEF1α-iCre-WPRE-bGHp(A)Virus constructsingle-stranded genome-
mTH-iCre-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-hsyn-UCN1.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV.rTH.PI.Cre.SV40Virus constructPI: Wilson-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

Reagents and Solutions
  • Pharmacological agents: Drugs and compounds used for anesthesia, analgesia, behavioral modulation, or pharmacological intervention (e.g., ketamine, buprenorphine, muscimol).
  • Physiological solutions: Buffers and fluids such as saline, PBS, or artificial cerebrospinal fluid (aCSF) used in perfusion, injections, and surgical preparation.
  • Virus constructs: Engineered viral vectors (e.g., AAV, lentivirus) used for delivering genetic material into neurons or glial cells.
  • Tracer dyes: Fluorescent or visible dyes like DiI, CTB, and Fast Green used for anatomical tracing, injection visualization, or tissue marking.
  • Antibodies and immunoreagents: Reagents used for labeling proteins and cell types in immunohistochemistry, such as primary/secondary antibodies and counterstains (e.g., DAPI).
  • Chemical reagents: Common lab chemicals like paraformaldehyde (PFA), Triton X-100, and EDTA used in tissue preparation, fixation, or signal amplification.
Device-Linked Consumables
  • Silicon probe designs: Microfabricated electrode arrays (e.g., Neuropixels, NeuroNexus) used for high-density extracellular electrophysiological recordings.
  • Single wire electrodes: Simple electrodes used for stimulation or recording from single brain regions or peripheral targets.
  • Optic fiber designs: Configurations of optic fibers for light delivery in optogenetics or signal collection in fiber photometry setups.
  • Optical components: Implantable optical elements such as GRIN lenses, microprisms, or cranial windows for in vivo imaging and optical access to the brain.
  • Consumable devices: Subject-mounted components like headstage baseplates, miniscope adapters, microdrives, or chronic interface mounts that are consumed per experiment or animal.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.