Consumables

pZac2.1 gfaABC1D-tdTomatoVirus constructPI: Khakh. Activity: Constitutive-
rAAV-CMV-CRE-plus-WPRE-bGH polyAVirus construct--
pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFPVirus constructActivity: Flp dependent. PI: Deisseroth-
pAV-EF1a-PGE2mutVirus constructTiter: ≥1*10E13vg/ml,50ul-
hU6-sgRNA(mBmal1-v1)-hU6-sgRNA(mBmal1-v3)-hSyn1-mCherry_KASH-hGHp(A)Virus constructsingle-stranded genome-
AAV9-Ef1a-DIO-5HT2MVirus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV9-c-fos-tTAVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
shortCAG-dlox-GCaMP6f(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-EF1a-vCreVirus constructPI: Deisseroth-
pAAV-syn-FLEX-axon-jYCaMP1sVirus constructPI: Podgorski. Activity: Cre dependent-
mDlx-HBB-chI-jGCaMP8m-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hEF1α-EBFP2-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-EBFP2_2A_DreO-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hSyn1-chI-IgK_TM_KA5(N-term.)_CaM_NES_TEV(N-term.)_AsLOV2_TEVsite_tTA-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-jGCaMP7s-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pGP-AAV-syn-jGCaMP8m-WPREVirus constructPI: GENIE. Activity: Constitutive-
FG365LECOptic fiber designØ365 µm Core, 0.22 NA, Low OH TECS Double-Clad Multimode Fiber-
rAAV-hSyn-DIO-5HT2.1(mutant)Virus construct--
hEF1α-dlox-eNpHR3.0_iRFP(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV-EF1a-DIO-JEDI-2P-Kv-WPREVirus constructPI: St-Pierre. Activity: Cre dependent-
hEF1α-jGCaMP7f-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-nEF1a-EGFP-3XFlag-WPRE-hGH polyAVirus construct--
AAV-CaMKII-hChR2(E123T/T159C)-p2A-mCherry-WPREVirus constructCell Filling Variants-
AAV5-CaMKIIa-DIO-mCherryVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
AAV9-EFS-DIO-CRF3.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
FT200UMTOptic fiber designØ200 µm Core, 0.39 NA, High OH TECS-Clad Multimode Fiber-
AAV9-hSyn-eNpHR3.0-EYFPVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
hEF1α-GCaMP6f-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-H2BJ_mScarlet-I-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV9-CaMKIIa-eNpHR3.0-EYFPVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
pAAV-hSyn-hM3D(Gq)-mCherryVirus constructCre requirement: none. PI: Roth-
rAAV-EF1α-DIO-ChETA-EYFP-WPRE-hGH polyAVirus construct--
pAAV-nEF-Coff/Fon-ChRmine-oScarletVirus constructActivity: Flp dependent. PI: Deisseroth-
AAV-CaMKII-hChR2(E123A)-mCherryVirus constructSecond-generation Ultrafast Optogenetic Control-
pAAV-CAG-dLight1.1Virus constructPI: Tian. Activity: Constitutive-
hSyn1-hChR2(H134R)_EYFP-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-dlox-GACh3.0(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-dFRT-GCaMP6m_mRpL10a(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-hSyn-DA4.4Virus construct--
hSyn1-FLPo-SV40p(A)Virus constructself-complementary genome-
rAAV-CMV-Mito-dsRed-P2A-CRE-WPRE-bGH polyAVirus construct--
PFD-rAAV-mDlx-DA2h-WPRE-hGH polyAVirus construct--
rAAV-hSyn-NLS-FLP-bGH polyAVirus construct--
mCaMKIIα-NES_jRCaMP1a-WPRE-bGHp(A)Virus constructsingle-stranded genome-
FZM05020Optic fiber designØ50 µm Core, 0.20 NA, ZBLAN Multimode Fiber-
hEF1α-Con/Fon(oG)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
PRS2(8x)-hDBH-chI-hChR2(H134R)_EYFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
1310BHPOptic fiber designSingle Mode Fiber, 1300 - 1625 nm, Ø125 µm Cladding-
pAAV-hSyn-JEDI-2P-Kv-WPREVirus constructPI: St-Pierre. Activity: Constitutive-
CAG-dFRT-IgK_3xHA_WGA2_mCherry(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

Reagents and Solutions
  • Pharmacological agents: Drugs and compounds used for anesthesia, analgesia, behavioral modulation, or pharmacological intervention (e.g., ketamine, buprenorphine, muscimol).
  • Physiological solutions: Buffers and fluids such as saline, PBS, or artificial cerebrospinal fluid (aCSF) used in perfusion, injections, and surgical preparation.
  • Virus constructs: Engineered viral vectors (e.g., AAV, lentivirus) used for delivering genetic material into neurons or glial cells.
  • Tracer dyes: Fluorescent or visible dyes like DiI, CTB, and Fast Green used for anatomical tracing, injection visualization, or tissue marking.
  • Antibodies and immunoreagents: Reagents used for labeling proteins and cell types in immunohistochemistry, such as primary/secondary antibodies and counterstains (e.g., DAPI).
  • Chemical reagents: Common lab chemicals like paraformaldehyde (PFA), Triton X-100, and EDTA used in tissue preparation, fixation, or signal amplification.
Device-Linked Consumables
  • Silicon probe designs: Microfabricated electrode arrays (e.g., Neuropixels, NeuroNexus) used for high-density extracellular electrophysiological recordings.
  • Single wire electrodes: Simple electrodes used for stimulation or recording from single brain regions or peripheral targets.
  • Optic fiber designs: Configurations of optic fibers for light delivery in optogenetics or signal collection in fiber photometry setups.
  • Optical components: Implantable optical elements such as GRIN lenses, microprisms, or cranial windows for in vivo imaging and optical access to the brain.
  • Consumable devices: Subject-mounted components like headstage baseplates, miniscope adapters, microdrives, or chronic interface mounts that are consumed per experiment or animal.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.