Consumables

rAAV-CaMKIIa-hChR2(H134R)-mCherry-WPRE-hGH polyAVirus construct--
rAAV-D1-fDIO-CRE-mCherry-WPRE-bGH polyAVirus construct--
rAAV-CaMkIIa-Tau-tdtomato-WPREsVirus construct--
pAAV-hsyn-Jaws-KGC-GFP-ER2Virus constructActivity: Constitutive. PI: Boyden-
hSyn1-chI-GreenGlifon4000-WPRE-bGHp(A)Virus constructsingle-stranded genome-
S5E2-HBB-chI-dTomato_2A_NLS_dTomato-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hSyn1-scFLARE2-bGHp(A)Virus constructsingle-stranded genome-
AAV1-EFS-DIO-TAC01Virus constructTiter: ≥1*10E13vg/ml,50ul-
AAV-Ef1a-DIO eNpHR 3.0-EYFPVirus constructActivity: Cre dependent. PI: Deisseroth-
AAV8-U6-shRNA(scramble)-CMV-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
mCaMKIIα-GCaMP6m_2A_ChRmine_MTS_Kv2.1-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV5-GfaABC1D-rATP0.5Virus constructTiter: ≥1*10E13vg/ml,50ul-
rAAV-hSyn-axon-GCaMP6f-WPRE-hGH polyAVirus construct--
PFD-rAAV-EF1a-fDIO-iGABASnFR-WPRE-hGH polyAVirus construct--
hSyn1-eOPN3_mScarlet-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-CMV-Mito-dsRed-WPRE-bGH polyAVirus construct--
FFH10026Optic fiber designØ100 µm Core, 0.26 NA, Double-Clad InF₃ Multimode Fiber-
rAAV-CAG-hChR2(H134R)-mCherry-nrxn1a rev-WPRE-hGH polyAVirus construct--
hSyn1-chI-IRES_mCitrine-WPRE-bGHp(A)Virus constructsingle-stranded genome-
shortCAG-dlox-hM4D(Gi)_mCherry(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
A1x32-7mm-100-1250Silicon probe designSpecial Order-
rAAV-RAM-d2TTA::TRE-ArchT-EGFP-WPRE-pAVirus construct--
AAV9-hSyn-OXT1.5Virus constructTiter: ≥1*10E13vg/ml,50ul-
GF3Optic fiber designPhotosensitive SM Fiber, 1500 - 1600 nm, 7.5 µm MFD, 0.16 NA-
pAAV.Syn.NES.jRCaMP1a.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Constitutive-
AAV-CAG-ArchT-GFPVirus constructNeural Silencers-
hEF1α-dFRT-EGFP_2A_FLAG_TeTxLC(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-hSyn_ATP1.1mVirus constructTiter: ≥1*10E13vg/ml,50ul-
AAV9-hSyn-gACh4mVirus constructTiter: ≥1*10E13vg/ml,50ul-
hSyn1-FLIP(c.-o.)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-TBG-hChR2-EGFP-WPRE-hGH polyAVirus construct--
hSyn1-ConVERGD(H2BJ_3xHA_2A_G (CVS-N2c))-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-CMV-DIO-EGFP-WPRE-hGH polyAVirus construct--
rAAV-CAG-DIO-EGFP-WPRE-hGH polyAVirus construct--
AAV8-ALB-CreVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
pAAV-hSyn-JEDI-2P-WPREVirus constructPI: St-Pierre. Activity: Constitutive-
hEF1α-lox2272mut-mCherry-loxPmut-DTA(rev)-dloxmut-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-Ki67-hChR2-EYFP-WPRE-hGH polyAVirus construct--
hGFAP-hHBbI/E-dlox-dTomato-EGFP(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
FT1000UMTOptic fiber designØ1000 µm Core, 0.39 NA, High OH TECS-Clad Multimode Fiber-
AAV5-GfaABC1D-ATP1.1mVirus constructTiter: ≥1*10E13vg/ml,50ul-
pAAV.CAG.Flex.GCaMP6m.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Cre dependent-
pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpAVirus constructCre requirement: Flp-dependent. PI: Gether-
hSyn1-ATeam1.03-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV-CaMKII-hChR2(T159C)-p2A-mCherry-WPREVirus constructCell Filling Variants-
pZac2.1 gfaABC1D-lck-GCaMP6fVirus constructPI: Khakh. Activity: Constitutive-
hSyn1-chI-dFRT-eArchT3.0_EYFP-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-GfaABC1D-hM3D(Gq)-mCherry-SV40 polyAVirus construct--
pAAV-EF1a-N-CretrcintGVirus constructPI: Cepko-
rAAV-EF1α-DIO-GRAB-NEmut-WPRE-hGH polyAVirus construct--

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

Reagents and Solutions
  • Pharmacological agents: Drugs and compounds used for anesthesia, analgesia, behavioral modulation, or pharmacological intervention (e.g., ketamine, buprenorphine, muscimol).
  • Physiological solutions: Buffers and fluids such as saline, PBS, or artificial cerebrospinal fluid (aCSF) used in perfusion, injections, and surgical preparation.
  • Virus constructs: Engineered viral vectors (e.g., AAV, lentivirus) used for delivering genetic material into neurons or glial cells.
  • Tracer dyes: Fluorescent or visible dyes like DiI, CTB, and Fast Green used for anatomical tracing, injection visualization, or tissue marking.
  • Antibodies and immunoreagents: Reagents used for labeling proteins and cell types in immunohistochemistry, such as primary/secondary antibodies and counterstains (e.g., DAPI).
  • Chemical reagents: Common lab chemicals like paraformaldehyde (PFA), Triton X-100, and EDTA used in tissue preparation, fixation, or signal amplification.
Device-Linked Consumables
  • Silicon probe designs: Microfabricated electrode arrays (e.g., Neuropixels, NeuroNexus) used for high-density extracellular electrophysiological recordings.
  • Single wire electrodes: Simple electrodes used for stimulation or recording from single brain regions or peripheral targets.
  • Optic fiber designs: Configurations of optic fibers for light delivery in optogenetics or signal collection in fiber photometry setups.
  • Optical components: Implantable optical elements such as GRIN lenses, microprisms, or cranial windows for in vivo imaging and optical access to the brain.
  • Consumable devices: Subject-mounted components like headstage baseplates, miniscope adapters, microdrives, or chronic interface mounts that are consumed per experiment or animal.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.