Consumables

hSyn1-chI-dLight1.1-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-hSyn-GCaMp6f-P2A-NLS-dTomato-WPRE-hGH polyAVirus construct--
rAAV-EF1α-DIO-taCasp3-TEVp-P2A-EGFP-WPRE-hGH polyAVirus construct--
hSyn1-chI-dlox-EGFP_hRAB5a(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hGFAP-chI[3x:sh(mGrin1)]-Lck_GCaMP6f-SV40p(A)Virus constructsingle-stranded genome-
AAV9-EFS-DIO-Ado1.0medVirus constructTiter: ≥1*10E13vg/ml, 50ul-
SM800-5.6-125Optic fiber designSingle Mode Fiber, 830 - 980 nm, Ø125 µm Cladding-
rAAV-D1-CRE-WPRE-hGH polyAVirus construct--
rAAV-EF1α-DIO-GRAB-NE1h-WPRE-hGH polyAVirus construct--
AAV-EF1a-DIO-hChR2(C128S/D156A)-EYFPVirus constructStabilized Step Function Opsins: SSFOs-
rAAV-CaMKIIa-Mito-dsRed-WPRE-hGH polyAVirus construct--
AAV-CamKIIa-jGCaMP8f-WPREVirus constructPI: Looger. Activity: Constitutive-
pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]Virus constructActivity: Cre dependent. PI: Boyden-
mscRE4-HBB-EGFP-WPRE-hGHpAVirus constructsingle-stranded genome-
CAG-NLS-GFPVirus constructPI: Gradinaru. Activity: Constitutive-
AAV-hSyn-FLEX-CoChR-GFPVirus constructNeural Activators - FLEX Chronos/Chrimson (no AAV6)-
PS-PM980Optic fiber design970 - 1550 nm PM Photosensitive Fiber, 0.12 NA, 6.6 µm MFD-
hSyn1-HA_hM3D(Gq)_IRES_mCitrine-WPRE-hGHp(A)Virus constructsingle-stranded genome-
FG200LEA (Ø 200/220µm, 0.22 NA)Optic fiber designØ200 µm Core Glass Clad Silica Multimode Optical Fiber, 0.22 NA-
hSyn1-dlox-CoChR_EGFP(rev)-dlox-WPRE-bGHp(A)-SV40p(A)Virus constructsingle-stranded genome-
A2x8-11mm-125-200-177Silicon probe designSpecial Order-
pAAV-syn-dLight1.3bVirus constructPI: Tian. Activity: Constitutive-
rAAV-mTH-NLS-CRE-WPRE-SV40 polyAVirus construct--
AAV9-CaMkIIa-GCaMP6sVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
AAV9-EFS-fDIO-eCB2.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV9-SST-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
rAAV-CMV-DIO-mCherry-tau-WPRE-hGH polyAVirus construct--
AAV-hSyn-Soma-jGCaMP8mVirus constructPI: Fyhn. Activity: Constitutive-
pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyAVirus constructPI: Feng. Activity: Constitutive-
rAAV-PTH-hM4D(Gi)-mCherry-WPRE-hGH polyAVirus construct--
rAAV-EF1α-DIO-NpHR3.0-EYFP-WPRE-hGH polyAVirus construct--
hSyn1-chI-dlox-H2BJ_EGFP(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-hSyn-GRAB_NEmutVirus constructPI: Li. Activity: Constitutive-
pAAV-synP-FLEX-splitTVA-EGFP-B19GVirus constructCan be used to complement deletion-mutant rabies virus. PI: Wickersham.-
hSyn1-chI-OxLight1-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-EF1α-fDIO-CRE-WPREsVirus construct--
rAAV-Ef1α-DIO-His-EGFP-2A-TVA-WPRE-hGH polyAVirus construct--
rAAV-CaMKIIa-GCaMP6s-P2A-NLS-dTomato-WPRE-bGH polyAVirus construct--
rAAV-EF1a-Con/Foff-hChR2(H134R)-EYFP-WPRE-hGH polyAVirus construct--
FG200LAOptic fiber designCoreless Termination Fiber, Ø200 µm Glass Rod-
hSyn1-dlox-EGFP(rev)-chI[4x:sh(NS)](rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV5-GfaABC1D-hM3D(Gq) -mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
rAAV-EF1α-DIO-GCaMp6m-WPRE-hGH polyAVirus construct--
AAV1-hsyn-TAC01Virus constructTiter: ≥1*10E13vg/ml,50ul-
AAV9-EF1a-DIO-hChR2(C128S/D156A)-EYFPVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
AAV2-CMV-intron-ACE2-FHVirus constructTiter: ≥5*10E12vg/ml. Volume: 50ul-
shortCAG-dLight1.3b-WPRE-SV40p(A)Virus constructsingle-stranded genome-
AAV5-GFaABC1D-DA3mVirus constructTiter: ≥1*10E13vg/ml,50ul-
hGFAP-hHBbI/E-chI[4x:sh(NS)]-EGFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hGFAP-hHBbI/E-Tq-Ca-FLITS-WPRE-bGHp(A)Virus constructsingle-stranded genome-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

Reagents and Solutions
  • Pharmacological agents: Drugs and compounds used for anesthesia, analgesia, behavioral modulation, or pharmacological intervention (e.g., ketamine, buprenorphine, muscimol).
  • Physiological solutions: Buffers and fluids such as saline, PBS, or artificial cerebrospinal fluid (aCSF) used in perfusion, injections, and surgical preparation.
  • Virus constructs: Engineered viral vectors (e.g., AAV, lentivirus) used for delivering genetic material into neurons or glial cells.
  • Tracer dyes: Fluorescent or visible dyes like DiI, CTB, and Fast Green used for anatomical tracing, injection visualization, or tissue marking.
  • Antibodies and immunoreagents: Reagents used for labeling proteins and cell types in immunohistochemistry, such as primary/secondary antibodies and counterstains (e.g., DAPI).
  • Chemical reagents: Common lab chemicals like paraformaldehyde (PFA), Triton X-100, and EDTA used in tissue preparation, fixation, or signal amplification.
Device-Linked Consumables
  • Silicon probe designs: Microfabricated electrode arrays (e.g., Neuropixels, NeuroNexus) used for high-density extracellular electrophysiological recordings.
  • Single wire electrodes: Simple electrodes used for stimulation or recording from single brain regions or peripheral targets.
  • Optic fiber designs: Configurations of optic fibers for light delivery in optogenetics or signal collection in fiber photometry setups.
  • Optical components: Implantable optical elements such as GRIN lenses, microprisms, or cranial windows for in vivo imaging and optical access to the brain.
  • Consumable devices: Subject-mounted components like headstage baseplates, miniscope adapters, microdrives, or chronic interface mounts that are consumed per experiment or animal.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.