Consumables

mCaMKIIα-jGCaMP8m-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-hDlx-Flex-GFP-Fishell_6Virus constructPI: Fishell. Activity: Cre dependent-
hSyn1-chI-rPSD-95_tagRFP-WPRE-SV40p(A)Virus constructsingle-stranded genome-
AAV9-hSyn-hM4D(Gi)-mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
FG050LGAOptic fiber designØ50 µm Core Glass Clad Silica Multimode Optical Fiber, 0.22 NA-
hSyn1-chI-dFRT-jGCaMP8s(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-NOPLight-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV.hSynap.iGABASnFRVirus constructPI: Looger. Activity: Constitutive-
rAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP-WPRE-hGH polyAVirus construct--
rAAV-TRE3G-DIO-CaMPARI-WPRE-hGHpAVirus construct--
FT300EMTOptic fiber designØ300 µm Core, 0.39 NA, Low OH TECS-Clad Multimode Fiber-
AAV8-CMV-beta globin-Cre-P2A-GFPVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
hEF1α-dlox-dLight1.1(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-D1-ChETA hChR2(E123T H134R)-mCherry-WPRE-bGH polyAVirus construct--
shortCAG-dlox-SF_Venus_iGluSnFR(A184S)(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-EF1α-pre-mGRASP-WPRE-hGH polyAVirus construct--
AAV9-CaMKIIa-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
hSyn1-chI-dlox-iGluSnFR3.v857.PDGFR(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Cre dependent-
hSyn1-CapChR2_mScarlet-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV-nEF-Con/Fon-ChR2-mCherryVirus constructActivity: Cre and Flp dependent. PI: Deisseroth-
hSyn1-GRAB(VIP1.0)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-TRE-NEmut(NE2.1mut)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
pAAV-hsyn-GRAB_5-HT1.0Virus constructPI: Li. Activity: Constitutive-
rAAV-TRE3G-DIO-mCherry-WPRE-hGH polyAVirus construct--
hEF1α-dlox-mTagBFP2(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
FG600LEA (Ø 600/660µm, 0.22 NA)Optic fiber designØ600 µm Core Glass Clad Silica Multimode Optical Fiber, 0.22 NA-
pAAV.Syn.Flex.GCaMP6f.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Cre dependent-
pAAV_hSyn1_nLightRVirus constructPI: Patriarchi. Activity: Constitutive-
AAV9-hsyn-DIO-NE2m(NE3.1)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
rAAV-EF1α-Con Fon-GCaMp6s-WPRE-hGH polyAVirus construct--
mCaMKIIα(short)-chI-mTagBFP2_2A_mSyp1_tdTomato-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-hSyn-FLP-EGFP-WPRE-hGH polyAVirus construct--
rAAV-nEf1α-mCherry-WPRE-hGH polyAVirus construct--
mPer2-chI-mPer2I-Venus_NLS_Pest-WPRE-bGHp(A)Virus constructsingle-stranded genome-
PFD-rAAV-VGAT1-axon-ACh3.0-WPREsVirus construct--
AAV-EF1a-DIO-ChR2(T159C)-mCherryVirus constructSecond-generation Ultrafast Optogenetic Control-
E29-HBB-chI-hChR2(H134R)_EGFP_2A_NLS_cdEGFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-E-SARE-Cre-ERT2-PEST-WPRE-hGH polyAVirus construct--
Human CRISPRa sgRNA library Calabrese in XPR_502Virus constructHuman CRISPRa activation library targeting 18,885 genes. 56,762 sgRNAs. Puromycin selection. PI: Doench, Root.-
AAV2 retro-CMV-beta globin-Cre-P2A-GFPVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
pAAV-EF1a-Flp-DOG-NWVirus constructPI: Cepko-
AAV-CAG-jGCaMP8f-WPREVirus constructPI: Looger. Activity: Constitutive-
rAAV-nEF1α-fDIO-taCasp3-TEVp-WPRE-hGH polyAVirus construct--
pAAV SYN1 HA-hM4D(Gi)Virus constructCre requirement: none. PI: Richie-
pAAV-Ef1a-mCherry-IRES-CreVirus constructPI: Deisseroth-
mCaMKIIα-eArchT3.0_EYFP-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2Virus constructPI: Looger. Activity: Constitutive-
hSyn1-dlox-HA_hKORD_IRES_mCyRFP1(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hDlx-HBB-chI-dlox-hM3D(Gq)_EGFP(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

Reagents and Solutions
  • Pharmacological agents: Drugs and compounds used for anesthesia, analgesia, behavioral modulation, or pharmacological intervention (e.g., ketamine, buprenorphine, muscimol).
  • Physiological solutions: Buffers and fluids such as saline, PBS, or artificial cerebrospinal fluid (aCSF) used in perfusion, injections, and surgical preparation.
  • Virus constructs: Engineered viral vectors (e.g., AAV, lentivirus) used for delivering genetic material into neurons or glial cells.
  • Tracer dyes: Fluorescent or visible dyes like DiI, CTB, and Fast Green used for anatomical tracing, injection visualization, or tissue marking.
  • Antibodies and immunoreagents: Reagents used for labeling proteins and cell types in immunohistochemistry, such as primary/secondary antibodies and counterstains (e.g., DAPI).
  • Chemical reagents: Common lab chemicals like paraformaldehyde (PFA), Triton X-100, and EDTA used in tissue preparation, fixation, or signal amplification.
Device-Linked Consumables
  • Silicon probe designs: Microfabricated electrode arrays (e.g., Neuropixels, NeuroNexus) used for high-density extracellular electrophysiological recordings.
  • Single wire electrodes: Simple electrodes used for stimulation or recording from single brain regions or peripheral targets.
  • Optic fiber designs: Configurations of optic fibers for light delivery in optogenetics or signal collection in fiber photometry setups.
  • Optical components: Implantable optical elements such as GRIN lenses, microprisms, or cranial windows for in vivo imaging and optical access to the brain.
  • Consumable devices: Subject-mounted components like headstage baseplates, miniscope adapters, microdrives, or chronic interface mounts that are consumed per experiment or animal.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.