Consumables

pAAV-CKIIa-stGtACR2-FusionRedVirus constructActivity: Constitutive. PI: Yizhar-
rAAV-mDLx-iGABASnFR-WPREsVirus construct--
hSyn1-chI-Tq-Ca-FLITS-WPRE-bGHp(A)Virus constructsingle-stranded genome-
Neuropixels 1.0 Probe - Phase 3B2Silicon probe design960 channels in total to select from1
rAAV-hCD68-CRE-WPRE-PAVirus construct--
AAV-PHP.EB-CaMKIIa-mCherryVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
AAV8-GfaABC1D-hM3D(Gq) -mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
hSyn1-chI-dFRT-GCaMP6f(rev)-dFRT-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-CaMKIIa-DIO-hM4D(Gi)-mCherry-WPRE-hGH polyAVirus construct--
AAV9-CAG-r5HT1.0Virus constructTiter: ≥1*10E13vg/ml,50ul-
hSyn1-chI-hERT2_Cre_hERT2-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-VGAT1-DIO-hM4D(Gi)-mCherry-WPRE-hGH polyAVirus construct--
rAAV-CaMKIIa-hM4D(Gi)-EGFP-WPRE-hGH polyAVirus construct--
pAAV-hSyn-GRAB_OTmutVirus constructPI: Li. Activity: Constitutive-
pBOB-CAG-SARS-CoV2-Spike-HAVirus constructExpresses SARS-CoV-2 Spike protein. CAG promoter. PI: Pao.-
PM780-HPOptic fiber designSingle Mode Polarization-Maintaining Fiber, 770 - 1100 nm, PANDA-
AAV-FLEX-Arch-GFPVirus constructActivity: Cre dependent. PI: Boyden-
hGFAP-chI-monSTIM1-bGHp(A)Virus constructsingle-stranded genome-
hGFAP-hHBbI/E-iCre-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-iCre-WPRE-SV40p(A)Virus constructsingle-stranded genome-
FP1500ERTOptic fiber design0.50 NA, Ø1500 µm Core Multimode Fiber, Low OH-
rAAV-CAG-DIO-mCherry-WPRE-hGH polyA-WPREs-pAVirus construct--
rAAV-GfaABC1D-ATP1.0Virus construct--
AAV9-EF1a-DIO-GCaMP6f-P2A-nls-dTomatoVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
rAAV-CaMKIIa-mito-NES-jRGECO1a-WPRE-hGH polyAVirus construct--
pGP-AAV-syn-FLEX-jGCaMP8s-WPREVirus constructPI: GENIE. Activity: Cre dependent-
hSyn1-hM3D(Gq)_mCherry-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-TRE3g-DIO-hChR2(H134R)-YFP-WPREsVirus construct--
hSyn1-chI-EBFP2_2A_FLPo-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hSyn1-dlox-GAP43_6xHis_mScarlet-I(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV-EF1a-FLEX-Chronos-GFPVirus constructNeural Activators - FLEX Chronos/Chrimson (no AAV6)-
hEF1α-dlox-JEDI[1P](rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-dlox-rM3D(Gs)_EGFP(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
F-seriesSilicon probe designCambridge Neurotech F-series, 64ch, 6 shank, poly 2, 200µm shank spacing-
AAV9-GfaABC1D-jGCaMP7s-P2A-nls-dTomatoVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
pAAV-hSynapsin1-FLEx-axon-GCaMP6sVirus constructPI: Tian. Activity: Cre dependent-
E1-16chSilicon probe designCambridge Neurotech E-series, 16ch, 1 shank, edge-
rAAV-CaMKIIa-hM4D(Gi)-mCherry-WPRE-hGH polyAVirus construct--
hEF1α-FLPo-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40Virus constructPI: Schreiter. Activity: Constitutive-
E1-32chSilicon probe designCambridge Neurotech E-series, 32ch, 2 shanks, edge, 250µm shank spacing-
A5x12-Poly2-5mm-20s-lin-160Silicon probe designNeuroNexus 64ch, 5 shanks, poly 2, 200µm shank spacing (A5x12-Poly2-5mm-20s-lin-160)-
FZM20020Optic fiber designØ200 µm Core, 0.20 NA, ZBLAN Multimode Fiber-
AAV1-hsyn-CCK2.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
FG400LEAOptic fiber designØ400 µm Core Glass Clad Silica Multimode Optical Fiber, 0.22 NA-
hSyn1-chI-dlox-EGFP_2A_FLPo-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
AAV2-retro-CAG-DIO-FlpVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
AAV9-hsyn-HA1.0h-mScarletVirus constructTiter: ≥1*10E13vg/ml, 50ul-
hEF1α/hTLV1-Coff/Fon(iC++_EYFP)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
GF4AOptic fiber designCladding Mode Offset Photosensitive Fiber, 1500 - 1600 nm, 4.0 µm MFD, 0.30 NA-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

Reagents and Solutions
  • Pharmacological agents: Drugs and compounds used for anesthesia, analgesia, behavioral modulation, or pharmacological intervention (e.g., ketamine, buprenorphine, muscimol).
  • Physiological solutions: Buffers and fluids such as saline, PBS, or artificial cerebrospinal fluid (aCSF) used in perfusion, injections, and surgical preparation.
  • Virus constructs: Engineered viral vectors (e.g., AAV, lentivirus) used for delivering genetic material into neurons or glial cells.
  • Tracer dyes: Fluorescent or visible dyes like DiI, CTB, and Fast Green used for anatomical tracing, injection visualization, or tissue marking.
  • Antibodies and immunoreagents: Reagents used for labeling proteins and cell types in immunohistochemistry, such as primary/secondary antibodies and counterstains (e.g., DAPI).
  • Chemical reagents: Common lab chemicals like paraformaldehyde (PFA), Triton X-100, and EDTA used in tissue preparation, fixation, or signal amplification.
Device-Linked Consumables
  • Silicon probe designs: Microfabricated electrode arrays (e.g., Neuropixels, NeuroNexus) used for high-density extracellular electrophysiological recordings.
  • Single wire electrodes: Simple electrodes used for stimulation or recording from single brain regions or peripheral targets.
  • Optic fiber designs: Configurations of optic fibers for light delivery in optogenetics or signal collection in fiber photometry setups.
  • Optical components: Implantable optical elements such as GRIN lenses, microprisms, or cranial windows for in vivo imaging and optical access to the brain.
  • Consumable devices: Subject-mounted components like headstage baseplates, miniscope adapters, microdrives, or chronic interface mounts that are consumed per experiment or animal.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.