Consumables

hGFAP-mTagBFP_iCre-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hCMV-hHBbI/E-dlox-SfRE_rCaM_Oki(rev)-dlox-hGHp(A)Virus constructsingle-stranded genome-
shortCAG-6xHis_LiLac-WPRE-SV40p(A)Virus constructsingle-stranded genome-
shortCAG-dlox-EGFP_KASH(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-nEF-ChRmine-mScarletVirus constructActivity: Constitutive. PI: Deisseroth-
rAAV-GFaABC1D-EGFP-WPRE-SV40 polyAVirus construct--
FG400UEP (Ø 400/440µm, 0.22 NA)Optic fiber designØ400 µm Core Polyimide-Coated Multimode Fibers-
pTight-dlox-tdTomato(rev)-dlox-WPRE-bGHp(A)-rp(A)-pNrRAM-d2tTA-SV40p(A)Virus constructsingle-stranded genome-
pAAV.hSynap-FLEX.SF-iGluSnFR.A184SVirus constructPI: Looger. Activity: Cre dependent-
FG025LJAOptic fiber designØ25 µm Core, 0.10 NA, Step-Index Multimode Optical Fiber-
rAAV-CAG-DA1m-WPRE-pAVirus construct--
FG125LAOptic fiber designCoreless Termination Fiber, Ø125 µm Glass Rod-
mCaMKIIα-dlox-jGCaMP7f(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-EF1a-DIO-hM3D(Gq)-EGFP-WPREsVirus construct--
hSyn1-chI-dFRT-FLPo(rev)-synTerminator(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-Grm6- EYFP-WPRE-hGH polyAVirus construct--
rAAV-hSyn-CheRiff-eGFP-WPRE-bGH polyAVirus construct--
mCaMKIIα-hChR2(H134R)_EYFP-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-dFRT-ChrimsonR_tdTomato(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hGLUT1-chI-EGFP-bGHp(A)Virus constructsingle-stranded genome-
mDlx-HBB-chI-GCaMP6f-WPRE-bGHp(A)Virus constructsingle-stranded genome-
mCaMKIIα(short)-(pre)eGRASP(p30; cyan)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hEF1α/hTLV1-dlox-GCaMP6s_2A_NLS_dTomato(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
YB800-20/125DC-PMOptic fiber designYtterbium-Doped LMA PM Active Fiber, Cladding-Pumped, Double-Clad-
AAV-hSyn-Con/Fon hChR2(H134R)-EYFP-WPREVirus constructINTRSECT (AAV5 & DJ)-
hSyn1-NES_jRCaMP1b-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-EF1α-DIO-Tau-mCherry-WPRE-hGH polyAVirus construct--
hGFAP-hHBbI/E-EGFP-bGHp(A)Virus constructsingle-stranded genome-
pAAV-CAG-miRFP680-P2A-GFPVirus construct--
AAV9-GfaABC1D-Ado1.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
pAAV-Ef1a-fDIO-tdTomatoVirus constructPI: Jensen. Activity: Flp dependent-
rAAV-hSyn-hM4D(Gi)-EGFP-WPRE-hGH polyAVirus construct--
AAV-nEF-Con/Fon hChR2(H134R)-EYFP-WPREVirus constructINTRSECT (AAV5 & DJ)-
AAV8-EF1a-DIO-hM3D(Gq)-mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
HI1060-J9Optic fiber designSingle Mode Fiber, 980 - 1650 nm, Ø900 µm Hytrel Jacket-
hEF1α-dlox-tTAc(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-CaMKIIa-EGFP-WPRE-hGH polyAVirus construct--
rAAV-Ef1α-EYFP-WPRE-hGH polyAVirus construct--
AAV9-hSyn-GCaMP6s-P2A-nls-dTomatoVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
hEF1α-lox71-mTagBFP_Far(rev)-loxKR3-lox66-EYFP_Far(rev)-loxKR3-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV SYN1 HA-hM3D(Gq)Virus constructCre requirement: none. PI: Richie-
P-20/400DCOptic fiber designPassive LMA Double-Clad Fiber, 20 µm Core, Matched to YB1200-20/400DC-
FG550UECOptic fiber designØ550 µm Core, 0.22 NA, High OH TECS Double-Clad Multimode Fiber-
pAAV-CAG-NIR-GECO2GVirus constructPI: Campbell. Activity: Constitutive-
hSyn1-dlox-GAP43_6xHis_mCherry(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hDlx-HBB-chI-dlox-HA_hKORD_mCyRFP1(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
GIF50DOptic fiber designØ50 µm Core Graded-Index Multimode Optical Fiber, 0.20 NA, OM3-
CASI-chI[4x(shSARS-CoV-2)]-EGFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
UM22-400 (Ø 400/440µm, 0.22 NA)Optic fiber designØ400 µm Core Polyimide-Coated Multimode Fibers-
hEF1α-dFRT-mTagBFP2(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-GFAP-hM4D(Gi)-mCherryVirus constructCre requirement: none. PI: Roth-
rAAV-EF1α-DIO-axon-GCaMP6s-WPRE-hGH polyAVirus construct--
AAV9-EFS-ATG-FRT-polyA-N79-FRT-iP2A-eCB2.0Virus constructTiter: ≥1*10E13vg/ml,50ul-
rAAV-CRH-DIO-EGFP-WPRE-hGH polyAVirus construct--
pAAV-hSyn-DIO-rM3D(Gs)-mCherryVirus constructCre requirement: Cre-dependent. PI: Roth-
hEF1α-dFRT-MMTS(COX4)_V5_dAPEX2(rev)-dFRT-WPRE-hGH(pA)Virus constructsingle-stranded genome-
hSyn1-dlox-hM4D(Gi)_mCherry(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hEF1α-EGFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-CMV-DIO-hM3D(Gq)-P2A-mCherry-hGH polyAVirus construct--
AAV9-hSyn-C1V1-TS-mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
hSyn1-chI-dlox-eArchT3.0_EGFP(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-hSyn-DIO-hM3D(Gq)-EGFP-WPREsVirus construct--
hGFAP-jGCaMP8m_2A_iCre-WPRE-bGHp(A)Virus constructsingle-stranded genome-
FT1500EMTOptic fiber designØ1500 µm Core, 0.39 NA, Low OH TECS-Clad Multimode Fiber-
rAAV-Grm6-CRE-WPRE-hGH polyAVirus construct--
pAAV.GfaABC1D.PI.Lck-GFP.SV40Virus constructPI: Khakh. Activity: Constitutive-
mCaMKIIα(short)-(pre)eGRASP(p30; yellow)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
PFD-rAAV-hSyn-iATPSnFR1.0-WPRE-hGH polyAVirus construct--
rAAV-EF1α-DIO-FLP-T2A-EGFP-WPRE-hGH polyAVirus construct--
hSyn1-chI-dlox-eArch3.0_EYFP(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrineVirus constructCre requirement: none. PI: Roth-
AAV-CaMKII-ArchT-GFPVirus constructNeural Silencers (no AAV6)-
TRE3G-chI-(pre)eGRASP(p32; yellow)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
FP400ERTOptic fiber design0.50 NA, Ø400 µm Core Multimode Fiber, Low OH-
PM-S405-XPOptic fiber designSingle Mode Polarization-Maintaining Fiber, 400 - 680 nm, PANDA-
hEF1α/hTLV1-Con/Foff(HA_hM3D(Gq))-WPRE-hGHp(A)Virus constructsingle-stranded genome-
PFD-rAAV-hSyn-ArcLight-S249-WPRE-hGH polyAVirus construct--
AAV-mDlx-jGCaMP8m-WPREVirus constructPI: Looger. Activity: Constitutive-
pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3Virus constructPI: GENIE. Activity: Constitutive-
pENN.AAV.cTNT.PI.eGFP.WPRE.rBGVirus constructPI: Wilson. Activity: Constitutive-
AAV-hSyn-Con/Fon EYFP-WPREVirus constructINTRSECT (AAV5 & DJ)-
AAV9-hsyn-DIO-OXT1.5Virus constructTiter: ≥1*10E13vg/ml,50ul-
AAV1/2-CMV-CBA-human-A53T-alpha-synuclein-WPRE-BGH...Virus constructTiter : >5x10^12 GC/mL-
AAV9-CaMkIIa-GCaMP6s-P2A-nls-dTomatoVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
rAAV-EF1α-DIO-hM4D(Gi)-mCherry-WPREsVirus construct--
AAV-hSyn-Ribo-jGCaMP8mVirus constructPI: Fyhn. Activity: Constitutive-
3xCore2(390m)-HBB-iAChSnFR(Venus)-WPRE3-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-JEDI[2P]-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-ConVERGD(G [SAD B19])-WPRE3-SV40p(A)Virus constructsingle-stranded genome-
rAAV-GAD67-DIO-hM4D(Gi)-mCherry-WPRE-hGH polyAVirus construct--
rAAV-VGAT1-eNpHR3.0-EYFP-WPRE-hGH polyAVirus construct--
pLenti-SV40-Puro-EF1a-RFPVirus constructTiter: 1x10^8 IU/mL-
AAV phSyn1(S)-tdTomato-WPREVirus constructPI: Zeng. Activity: Constitutive-
AAV9-EF1a-DIO-jGCaMP7f-P2A-nls-dTomatoVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
UM22-200 (Ø 200/220µm, 0.22 NA)Optic fiber designØ200 µm Core Polyimide-Coated Multimode Fibers-
hEF1α-dlox-FLAG_TeTxLC(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hCMV-6xHis_T7tag_TEVsite_cjBlue2-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Cre dependent-
Buzsaki16Silicon probe designdistance between the first two sites: 20µm-
AAV-EF1a-DIO-ChR2(E123A)-EYFPVirus constructSecond-generation Ultrafast Optogenetic Control-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

  • Optic fiber designs: Thin, flexible fibers used for transmitting light signals, crucial for optical neural imaging and optogenetic manipulation of neurons.
  • Silicon probe designs: Microfabricated devices designed for recording or stimulating neural activity, allowing for high-resolution interfacing with neural tissue.
  • Virus constructs: Genetically engineered viruses used for delivering genes into neurons, enabling the study of gene function or the manipulation of neural activity.
  • Wire electrodes: Thin metal wires used for recording electrical signals from or delivering electrical stimulation to neurons, essential for electrophysiological studies.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.