Consumables

hSyn1-chI-dlox-GCaMP6mXC(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hEF1α-dlox-hChR2(C128S/D156A)_EYFP(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-SST-mCherry-WPRE-bGH polyAVirus construct--
AAV9-CAG-cyto-AdoHVirus constructTiter: ≥1*10E13vg/ml,50ul-
mPGK1-hHBbI/E-iCre-hGHp(A)Virus constructsingle-stranded genome-
hEF1α-dFRT-NES_jRCaMP1a(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hGFAP-hHBbI/E-GCaMP6s-bGHp(A)Virus constructsingle-stranded genome-
pAAV-FLEX-tdTomatoVirus constructPI: Boyden. Activity: Cre dependent-
rAAV-VGAT2-CRE-mCherry-WPRE-hGH polyAVirus construct--
pTight-dlox-dTomato(rev)-dlox-WPRE-bGHp(A)-rp(A)-pRAM-d2tTA-SV40p(A)Virus constructsingle-stranded genome-
mCaMKIIα-hChR2(H134R)_mCherry-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-hSyn-CRF2.0Virus constructTiter: ≥1*10E13vg/ml,50ul-
AAV-CaMKII-hChR2(E123T/T159C)-p2A-EYFP-WPREVirus constructCell Filling Variants-
mCaMKIIα-SomaGCaMP7f-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-STVirus constructPI: Histed. Activity: Cre-dependent-
mDlx-HBB-chI[4x:sh(NS)]-EGFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV9-hsyn-5HT2MVirus constructTiter: ≥1*10E13vg/ml, 50ul-
hSyn1-dFRT-mCherry(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-TRE3G-DIO-eArch3.0-EYFP-WPRE-hGHpAVirus construct--
AAV9-EF1a-DIO-eArch3.0-EYFPVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
pENN.AAV.CamKII.GCaMP6f.WPRE.SV40Virus constructPI: Wilson. Activity: Constitutive-
hSyn1-chI-dlox-hChR2(H134R)_rKv2.1_2A_Voltron2-ST(rev)-dlox-WPRE3-bGHp(A)Virus constructsingle-stranded genome-
pLenti-SV40-Puro-EF1a-LuciferaseVirus constructTiter: 1x10^8 IU/mL-
AAV-EF1a-BbChTVirus constructPI: Sanes. Activity: Cre dependent-
mCaMKIIα(short)-(pre)eGRASP(p32;green)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI[4x:sh(NS)]-mScarlet-I-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-EF1α-DIO-NES-jRCaMP1b-WPRE-hGH polyAVirus construct--
hChAT-eArchT3.0_mKate2-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-dlox-mCherry(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV5-GfaABC1D-Cyto-Ado2mVirus constructTiter: ≥1*10E13vg/ml,50ul-
pAAV-nEF-Con/Foff 2.0-ChRmine-oScarletVirus constructActivity: Cre dependent. PI: Deisseroth-
PRS2(8x)-hDBH-chI-iCre-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV9-EF1a-S1P2.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
shortCAG-mTurquoise2-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hEF1α/hTLV1-drox-EGFP_mRpL10a(rev)-drox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
SM600Optic fiber designSingle Mode Fiber, 633 - 780 nm, Ø125 µm Cladding-
rAAV-hSyn-GCaMP6m-Xc-WPRE-hGH polyAVirus construct--
hSyn1-ReaChR_mCitrine-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-RAM-d2tTA::TRE-EGFP-WPREpAVirus construct--
rAAV-Dlx5/6-hM3D(Gq)-mCherry-WPRE-hGH polyAVirus construct--
hSyn1-dlox-GRAB(NE2m)(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV.CAG.GCaMP6m.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Constitutive-
AAV-hSyn-mCherry-P2A-Cre-WPREVirus constructPI: Yang-
rAAV-hSyn-His-EGFP-2A-TVA-WPRE-hGH polyAVirus construct--
shortCAG-dlox-GAP43_6xHis_mNeonGreen(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-dlox-mCherry(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hEF1α-dlox-GCaMP6f_2A_NLS_dTomato(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV9-hSyn-rACh1.7Virus constructTiter: ≥1*10E13vg/ml,50ul-
hSyn1-chI-dlox-H2BJ_mScarlet-I-NLS_EGFP(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
AAV-EF1a-DIO-C1V1(t/t)-TS-p2A-EYFP-WPREVirus constructCell Filling Variants-
hSyn1-chI-dLight1.1-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-hSyn-GCaMp6f-P2A-NLS-dTomato-WPRE-hGH polyAVirus construct--
rAAV-EF1α-DIO-taCasp3-TEVp-P2A-EGFP-WPRE-hGH polyAVirus construct--
hSyn1-chI-dlox-EGFP_hRAB5a(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hGFAP-chI[3x:sh(mGrin1)]-Lck_GCaMP6f-SV40p(A)Virus constructsingle-stranded genome-
AAV9-EFS-DIO-Ado1.0medVirus constructTiter: ≥1*10E13vg/ml, 50ul-
SM800-5.6-125Optic fiber designSingle Mode Fiber, 830 - 980 nm, Ø125 µm Cladding-
rAAV-D1-CRE-WPRE-hGH polyAVirus construct--
rAAV-EF1α-DIO-GRAB-NE1h-WPRE-hGH polyAVirus construct--
AAV-EF1a-DIO-hChR2(C128S/D156A)-EYFPVirus constructStabilized Step Function Opsins: SSFOs-
rAAV-CaMKIIa-Mito-dsRed-WPRE-hGH polyAVirus construct--
AAV-CamKIIa-jGCaMP8f-WPREVirus constructPI: Looger. Activity: Constitutive-
pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]Virus constructActivity: Cre dependent. PI: Boyden-
mscRE4-HBB-EGFP-WPRE-hGHpAVirus constructsingle-stranded genome-
CAG-NLS-GFPVirus constructPI: Gradinaru. Activity: Constitutive-
AAV-hSyn-FLEX-CoChR-GFPVirus constructNeural Activators - FLEX Chronos/Chrimson (no AAV6)-
PS-PM980Optic fiber design970 - 1550 nm PM Photosensitive Fiber, 0.12 NA, 6.6 µm MFD-
hSyn1-HA_hM3D(Gq)_IRES_mCitrine-WPRE-hGHp(A)Virus constructsingle-stranded genome-
FG200LEA (Ø 200/220µm, 0.22 NA)Optic fiber designØ200 µm Core Glass Clad Silica Multimode Optical Fiber, 0.22 NA-
hSyn1-dlox-CoChR_EGFP(rev)-dlox-WPRE-bGHp(A)-SV40p(A)Virus constructsingle-stranded genome-
A2x8-11mm-125-200-177Silicon probe designSpecial Order-
pAAV-syn-dLight1.3bVirus constructPI: Tian. Activity: Constitutive-
rAAV-mTH-NLS-CRE-WPRE-SV40 polyAVirus construct--
AAV9-CaMkIIa-GCaMP6sVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
AAV9-EFS-fDIO-eCB2.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV9-SST-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
rAAV-CMV-DIO-mCherry-tau-WPRE-hGH polyAVirus construct--
AAV-hSyn-Soma-jGCaMP8mVirus constructPI: Fyhn. Activity: Constitutive-
pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyAVirus constructPI: Feng. Activity: Constitutive-
rAAV-PTH-hM4D(Gi)-mCherry-WPRE-hGH polyAVirus construct--
rAAV-EF1α-DIO-NpHR3.0-EYFP-WPRE-hGH polyAVirus construct--
hSyn1-chI-dlox-H2BJ_EGFP(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-hSyn-GRAB_NEmutVirus constructPI: Li. Activity: Constitutive-
pAAV-synP-FLEX-splitTVA-EGFP-B19GVirus constructCan be used to complement deletion-mutant rabies virus. PI: Wickersham.-
hSyn1-chI-OxLight1-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-EF1α-fDIO-CRE-WPREsVirus construct--
rAAV-Ef1α-DIO-His-EGFP-2A-TVA-WPRE-hGH polyAVirus construct--
rAAV-CaMKIIa-GCaMP6s-P2A-NLS-dTomato-WPRE-bGH polyAVirus construct--
rAAV-EF1a-Con/Foff-hChR2(H134R)-EYFP-WPRE-hGH polyAVirus construct--
FG200LAOptic fiber designCoreless Termination Fiber, Ø200 µm Glass Rod-
hSyn1-dlox-EGFP(rev)-chI[4x:sh(NS)](rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV5-GfaABC1D-hM3D(Gq) -mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
rAAV-EF1α-DIO-GCaMp6m-WPRE-hGH polyAVirus construct--
AAV1-hsyn-TAC01Virus constructTiter: ≥1*10E13vg/ml,50ul-
AAV9-EF1a-DIO-hChR2(C128S/D156A)-EYFPVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
AAV2-CMV-intron-ACE2-FHVirus constructTiter: ≥5*10E12vg/ml. Volume: 50ul-
shortCAG-dLight1.3b-WPRE-SV40p(A)Virus constructsingle-stranded genome-
AAV5-GFaABC1D-DA3mVirus constructTiter: ≥1*10E13vg/ml,50ul-
hGFAP-hHBbI/E-chI[4x:sh(NS)]-EGFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hGFAP-hHBbI/E-Tq-Ca-FLITS-WPRE-bGHp(A)Virus constructsingle-stranded genome-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

  • Optic fiber designs: Thin, flexible fibers used for transmitting light signals, crucial for optical neural imaging and optogenetic manipulation of neurons.
  • Silicon probe designs: Microfabricated devices designed for recording or stimulating neural activity, allowing for high-resolution interfacing with neural tissue.
  • Virus constructs: Genetically engineered viruses used for delivering genes into neurons, enabling the study of gene function or the manipulation of neural activity.
  • Wire electrodes: Thin metal wires used for recording electrical signals from or delivering electrical stimulation to neurons, essential for electrophysiological studies.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.