Consumables

hSyn1-GRAB(DA1h)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-Oxytocin-CRE-WPREsVirus construct--
rAAV-CaMKIIa-OChIEF(E163A T199C)-P2A-mCherry-WPRE-hGH polyAVirus construct--
PFD-rAAV-CaMKIIa-rGRABDA1h-WPRE-hGH polyAVirus construct--
rAAV-EF1a-NpHR3.0-EYFP-WPRE-hGH polyAVirus construct--
mDlx-HBB-chI-HA_hKORD_IRES_mCitrine-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV1-hSyn-TAC0.1Virus constructTiter: ≥1*10E13vg/ml, 50ul-
rAAV-TRE3g-CMV-H2B-mCherry-pAVirus construct--
AAV9-hsyn-r5HT2.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
pAAV-GFAP104-mCherryVirus constructPI: Boyden. Activity: Constitutive-
hSyn1-SF_iGluSnFR(A184S)-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hSyn1-dlox-TeTxLC_2A_NLS_dTomato(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV.GFAP.Cre.WPRE.hGHVirus constructPI: Wilson-
rAAV-Cfos-mCherry -WPRE-hGHpAVirus construct--
FG400LE (Ø 400/440µm, 0.22 NA)Optic fiber designØ400 µm Core Polyimide-Coated Multimode Fibers-
AAV9-hSyn-mCherryVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
mCaMKIIα-dlox-mCherry(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
FG400UEA (Ø 400/440µm, 0.22 NA)Optic fiber designØ400 µm Core Glass Clad Silica Multimode Optical Fiber, 0.22 NA-
rAAV-CaMKIIa-taCasp3-T2A-TEVp-WPRE-hGH polyAVirus construct--
AAV9-hSyn-NES-jRCaMP1b-WPREVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
ZFbs-mCaMKIIα-mRuby2_FingR(Gephyrin)_IL2RGTC-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV9-hSyn-bDA1.1Virus constructTiter: ≥1*10E13vg/ml,50ul-
hSyn1-dlox-hM4D(Gi)_mCherry-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
dCAS9-VP64_GFPVirus constructdCAS9-VP64 activator with GFP. No resistance. PI: Zhang.-
pTight-eNpHR3.0_EYFP-WPRE-bGHp(A)-pRAM-d2tTA-SV40p(A)Virus constructsingle-stranded genome-
rAAV-CMV-GCaMp6s-WPRE-bGH polyAVirus construct--
pAAV-Syn-Archon1-KGC-GFP-ER2Virus constructPI: Boyden. Activity: Constitutive-
mDlx-HBB-chI-NES_jRGECO1a-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pAAV-EF1a-mCherry-IRES-FlpoVirus constructPI: Deisseroth-
hU6-sgRNA(LacZ-v1)-hU6-sgRNA(LacZ-v2)-hSyn1-mCherry_KASH-hGHp(A)Virus constructsingle-stranded genome-
rAAV-nEf1α-DIO-EGFP-2A-TVA-2A-RVG -WPREsVirus construct--
shortCAG-dlox-GCaMP6s_2A_mBeRFP(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pZac2.1-GfaABC1D-lck-jGCaMP8sVirus constructPI: Looger. Activity: Constitutive-
pAAV-CAG-FLEx-iSeroSnFRVirus constructPI: Tian. Activity: Cre dependent-
rAAV-CAG-CRE-WPRE-hGH polyAVirus construct--
rAAV-OT-GCaMp6s-WPREsVirus construct--
rAAV-nEF1α-fDIO-hChR2(H134R)-EYFP-WPRE-hGH polyAVirus construct--
Buzsaki64Silicon probe designNeuroNexus 64ch, 8 shanks, staggered, buzsaki-style, 200µm shank spacing (Buzsaki64)-
rAAV-hSyn-DIO-GACh3.0-WPRE-hGH polyAVirus construct--
SM980G80Optic fiber designSingle Mode Fiber, 980 - 1550 nm, Ø80 µm Cladding-
SM800G80Optic fiber designSingle Mode Fiber, 830 - 980 nm, Ø80 µm Cladding-
hSyn1-dlox-TVA_2A_EGFP(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hEF1α/hTLV1-dlox-ChrimsonR_tdTomato(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
PFD-rAAV-Chat-axon-ACh3.0-WPREsVirus construct--
rAAV-VGAT2-EYFP-WPRE-hGH polyAVirus construct--
hEF1α/hTLV1-dFRT-EGFP_mRpL10a(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
P2-32chSilicon probe design<p>Cambridge Neurotech P-series, 32ch, 2 shanks, poly 2, 250µm shank spacing</p>-
pLenti-SV40-Puro-EF1a-CreVirus constructTiter: 1x10^8 IU/mL-
rAAV-hSyn-NES-jRGECO1a-WPRE-hGH polyAVirus construct--
rAAV-E-SARE-CreERT2-PEST-WPRE-hGH polyAVirus construct--
pZac2.1 gfaABC1D-tdTomatoVirus constructPI: Khakh. Activity: Constitutive-
rAAV-CMV-CRE-plus-WPRE-bGH polyAVirus construct--
pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFPVirus constructActivity: Flp dependent. PI: Deisseroth-
pAV-EF1a-PGE2mutVirus constructTiter: ≥1*10E13vg/ml,50ul-
hU6-sgRNA(mBmal1-v1)-hU6-sgRNA(mBmal1-v3)-hSyn1-mCherry_KASH-hGHp(A)Virus constructsingle-stranded genome-
AAV9-Ef1a-DIO-5HT2MVirus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV9-c-fos-tTAVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
shortCAG-dlox-GCaMP6f(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-EF1a-vCreVirus constructPI: Deisseroth-
pAAV-syn-FLEX-axon-jYCaMP1sVirus constructPI: Podgorski. Activity: Cre dependent-
mDlx-HBB-chI-jGCaMP8m-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hEF1α-EBFP2-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-EBFP2_2A_DreO-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hSyn1-chI-IgK_TM_KA5(N-term.)_CaM_NES_TEV(N-term.)_AsLOV2_TEVsite_tTA-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-jGCaMP7s-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pGP-AAV-syn-jGCaMP8m-WPREVirus constructPI: GENIE. Activity: Constitutive-
FG365LECOptic fiber designØ365 µm Core, 0.22 NA, Low OH TECS Double-Clad Multimode Fiber-
rAAV-hSyn-DIO-5HT2.1(mutant)Virus construct--
hEF1α-dlox-eNpHR3.0_iRFP(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV-EF1a-DIO-JEDI-2P-Kv-WPREVirus constructPI: St-Pierre. Activity: Cre dependent-
hEF1α-jGCaMP7f-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-nEF1a-EGFP-3XFlag-WPRE-hGH polyAVirus construct--
AAV-CaMKII-hChR2(E123T/T159C)-p2A-mCherry-WPREVirus constructCell Filling Variants-
AAV5-CaMKIIa-DIO-mCherryVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
AAV9-EFS-DIO-CRF3.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
FT200UMTOptic fiber designØ200 µm Core, 0.39 NA, High OH TECS-Clad Multimode Fiber-
AAV9-hSyn-eNpHR3.0-EYFPVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
hEF1α-GCaMP6f-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-H2BJ_mScarlet-I-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV9-CaMKIIa-eNpHR3.0-EYFPVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
pAAV-hSyn-hM3D(Gq)-mCherryVirus constructCre requirement: none. PI: Roth-
rAAV-EF1α-DIO-ChETA-EYFP-WPRE-hGH polyAVirus construct--
pAAV-nEF-Coff/Fon-ChRmine-oScarletVirus constructActivity: Flp dependent. PI: Deisseroth-
AAV-CaMKII-hChR2(E123A)-mCherryVirus constructSecond-generation Ultrafast Optogenetic Control-
pAAV-CAG-dLight1.1Virus constructPI: Tian. Activity: Constitutive-
hSyn1-hChR2(H134R)_EYFP-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-dlox-GACh3.0(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-dFRT-GCaMP6m_mRpL10a(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-hSyn-DA4.4Virus construct--
hSyn1-FLPo-SV40p(A)Virus constructself-complementary genome-
rAAV-CMV-Mito-dsRed-P2A-CRE-WPRE-bGH polyAVirus construct--
PFD-rAAV-mDlx-DA2h-WPRE-hGH polyAVirus construct--
rAAV-hSyn-NLS-FLP-bGH polyAVirus construct--
mCaMKIIα-NES_jRCaMP1a-WPRE-bGHp(A)Virus constructsingle-stranded genome-
FZM05020Optic fiber designØ50 µm Core, 0.20 NA, ZBLAN Multimode Fiber-
hEF1α-Con/Fon(oG)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
PRS2(8x)-hDBH-chI-hChR2(H134R)_EYFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
1310BHPOptic fiber designSingle Mode Fiber, 1300 - 1625 nm, Ø125 µm Cladding-
pAAV-hSyn-JEDI-2P-Kv-WPREVirus constructPI: St-Pierre. Activity: Constitutive-
CAG-dFRT-IgK_3xHA_WGA2_mCherry(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

  • Optic fiber designs: Thin, flexible fibers used for transmitting light signals, crucial for optical neural imaging and optogenetic manipulation of neurons.
  • Silicon probe designs: Microfabricated devices designed for recording or stimulating neural activity, allowing for high-resolution interfacing with neural tissue.
  • Virus constructs: Genetically engineered viruses used for delivering genes into neurons, enabling the study of gene function or the manipulation of neural activity.
  • Wire electrodes: Thin metal wires used for recording electrical signals from or delivering electrical stimulation to neurons, essential for electrophysiological studies.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.