Consumables

AAV9-sparse-5-HT1.0(5-HT2.1)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
pAAV-CAG-tTAVirus constructExpression System: CAG-driven, constitutive. Activity: Expression of the tet-off transactivator (tTA). PI: Gradinaru-
AAV2 retro-CMV-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
AAV9-hsyn-CCK2.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
rAAV-CMV-DIO-EGFP-2A-TK-WPRE-hGH polyAVirus construct--
hSyn1-jGCaMP8s_mRpL10a-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-TRE-fDIO-eCB2.0-IRES-tTAVirus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV-hSyn-Flex-Jaws-KGC-GFP-ER2(AAV8 only currently)Virus constructNeural Silencers - Jaws (Halo57) (no AAV6)-
SMF-28-J9Optic fiber designSingle Mode Fiber, 1260 - 1625 nm, Ø900 µm Hytrel Jacket-
hEF1α-YCNano140-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-GFAP-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
AAV-CAG-jGCaMP8m-WPREVirus constructPI: Looger. Activity: Constitutive-
pZac2.1-GfaABC1D-lck-jGCaMP8mVirus constructPI: Looger. Activity: Constitutive-
rAAV-hSyn-DIO-His-EGFP-2A-TVA -WPRE-hGH polyAVirus construct--
hSyn1-chI-mTagBFP2_2A_mSyp1_tdTomato-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-NLS_EGFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-hSyn-mGFP-T2A-Synaptophysin-mRuby-WPRE-hGH polyAVirus construct--
pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3Virus constructPI: GENIE. Activity: Constitutive-
{in progress}Virus constructsingle-stranded genome-
rAAV-Ef1α-DIO-EGFP-2A-cmTK-WPRE-hGH polyAVirus construct--
S630-HPOptic fiber designSingle Mode Fiber, 630 - 860 nm, Ø125 µm Cladding-
rAAV-EFS-DIO-OXT1.0-WPRE-hGH polyAVirus construct--
rAAV- GfaABC1D-cyto-GCaMP6f-SV40 polyAVirus construct--
AAV2 retro-hSyn-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
mCaMKIIα-ChrimsonR_mCherry-WPRE-hGHp(A)Virus constructsingle-stranded genome-
A8x1-tet-2mm-200-121Silicon probe design*This probe has an non-uniform layout. See company catalog for more information: 2018ProbeCatalog_20180515_Web, p.52 Another site with an area of 4200µm^2 is located on the fourth shank from the left, 500µm above the tetrode-
Morris CellTag Barcode Library Version 3Virus construct5,737 barcodes for single-cell clonal analysis. Expresses EGFP. PI: Morris.-
lentiCas9-BlastVirus constructExpresses S. pyogenes Cas9. Blasticidin resistance. EFS promoter. PI: Zhang.-
pAAV-Ef1a-fDIO-ChrimsonR-tdTomatoVirus constructActivity: Flp dependent. PI: Jensen-
AAV9-TRE3G-mCherry-WPRE-PAVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
rAAV-EF1α-DIO-FLP-plus-WPRE-hGH polyAVirus construct--
rAAV-RK-EGFP-WPRE-hGH polyAVirus construct--
AAV9-hSyn-CRF3.0-mScarletVirus constructTiter: ≥1*10E13vg/ml, 50ul-
rAAV-Hypocretin-CRE-WPRE-hGH polyAVirus construct--
rAAV-CaMKIIa -DIO-hM3D(Gq)-mCherry-WPRE-hGH polyAVirus construct--
rAAV-hSyn-dLight1.2-WPRE-pAVirus construct--
AAV9-hSyn-GCaMP6sVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
hGFAP-HA_hM3D(Gq)_IRES_mCitrine-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-dlox-RCaMP1.07(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-VGAT1-CRE-WPRE-hGH polyAVirus construct--
mCaMKIIα(short)-(pre)eGRASP(p30; green)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-dlox-mCherry_2A_FLPo(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Cre dependent-
pENN.AAV.CB7.CI.eGFP.WPRE.rBGVirus constructPI: Wilson. Activity: Constitutive-
pAAV-Ef1a-oScarletVirus constructPI: Deisseroth. Activity: Constitutive-
pAAV-mDlx-ChR2-mCherry-Fishell-3Virus constructActivity: Constitutive. PI: Fishell-
hEF1α-iCre-WPRE-bGHp(A)Virus constructsingle-stranded genome-
mTH-iCre-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-hsyn-UCN1.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV.rTH.PI.Cre.SV40Virus constructPI: Wilson-
AAV-hSyn-FLEX-ChrimsonR-tdTomatoVirus constructNeural Activators - FLEX Chronos/Chrimson (no AAV6)-
hSyn1-chI-dFRT-EYFP(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hGFAP-JEDI[2P]-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-hSyn-GRAB-RDA1m-WPRE-hGH polyAVirus construct--
hSyn1-dlox-ChrimsonR_tdTomato-dlox-WPRE-bGHp(A)-SV40p(A)Virus constructsingle-stranded genome-
630HPOptic fiber designSingle Mode Fiber, 600 - 770 nm, Ø125 µm Cladding-
rAAV-CX3CR1-nEF1a-EGFP-WPRE-hGH polyAVirus construct--
rAAV-EF1α-DIO-oChIEF(E163A/T199C)-P2A-dTomato-WPRE-bGH polyAVirus construct--
mDlx-HBB-chI-hChR2(H134R)_mCherry-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-CAG-fDIO-hChR2-EYFP-WPRE-hGH polyAVirus construct--
pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40Virus constructPI: Wilson-
rAAV-CaMKIIa-pre-mGRASP-2A-Histone-BFP-WPRE-hGH polyAVirus construct--
pAAV-CaMKIIa-hChR2(H134R)-mCherryVirus constructActivity: Constitutive. PI: Deisseroth-
rTH-chI-iCre-hGHp(A)Virus constructsingle-stranded genome-
rAAV-CAG-FLEX-hChR2(H134R)T2A-hM4D(Gi)-WPRE-SV40 polyAVirus construct--
hEF1α-dlox-(pro)taCasp3_2A_TEVp(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-hSyn-NTSmutVirus constructTiter: ≥1*10E13vg/ml,50ul-
hSyn1-chI-loxP-EGFP-loxP-SV40p(A)Virus constructself-complementary genome-
mCaMKIIα-EBFP2-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-CaMKII-CRFmutVirus constructTiter: ≥1*10E13vg/ml, 50ul-
hCMV-chI-dlox-mRuby3(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
CAG-mScarlet-I_2A-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-mCherry_2A_FLPo-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hCMV-chI-GreenGlifon600-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV.Syn.NES-jRCaMP1b.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Constitutive-
AAV9-CMV-beta globin-Cre-P2A-GFPVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
rAAV- GfaABC1D-hM4D-mCherry-SV40 polyAVirus construct--
rAAV-hSyn-DIO-RVG-WPRE-hGH polyAVirus construct--
AAV-CaMKIIa-EYFPVirus constructControl Fluorophores-
AAV2-CAG-mCherryVirus constructTiter: ≥5*10E12vg/ml Volume: 50ul-
hSyn1-dFRT-hM4D(Gi)_mCherry(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-GRAB(5-HT1.0)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-hSyn-Con/Fon-hChR2(H134R)-EYFP-WPRE-hGH polyAVirus construct--
AAV9-hsyn-mApple-CAAXVirus constructTiter: ≥1*10E13vg/ml,50ul-
hGFAP-mCherry-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-D2P-DsRed-WPRE-bGH polyAVirus construct--
hEF1α-dFRT-EGFP(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
P-10/125DCOptic fiber designPassive LMA Double-Clad Fiber, 10 µm Core, Matched to YB1200-10/125DC-
SM1250G80Optic fiber designSingle Mode Fiber, 1310 - 1550 nm, Ø80 µm Cladding-
pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpAVirus constructCre requirement: Flp-dependent. PI: Gether-
rAAV-GFaABC1D-EGFP-SV40 polyAVirus construct--
FT300UMTOptic fiber designØ300 µm Core, 0.39 NA, High OH TECS-Clad Multimode Fiber-
rAAV-hCD68-EYFP-WPRE-PAVirus construct--
hGFAP-hHBbI/E-GCaMP6f-bGHp(A)Virus constructsingle-stranded genome-
hCMV-SaCas9-bGHp(A)-hU6-sgRNA(control)Virus constructsingle-stranded genome-
HB800GOptic fiber designSingle Mode Polarization-Maintaining Fiber, 830 nm, Bend-Insensitive Bow-Tie-
AAV9-EF1a-EP2.0/AAV-EF1a-PGE2-1.0Virus constructTiter: ≥1*10E13vg/ml,50ul-
rTH-hChR2(H134R)_EYFP-hGHp(A)Virus constructsingle-stranded genome-
pGP-AAV-syn-FLEX-jGCaMP8m-WPREVirus constructPI: GENIE. Activity: Cre dependent-
hEF1α-RCaMP1.07-WPRE-hGHp(A)Virus constructsingle-stranded genome-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

  • Optic fiber designs: Thin, flexible fibers used for transmitting light signals, crucial for optical neural imaging and optogenetic manipulation of neurons.
  • Silicon probe designs: Microfabricated devices designed for recording or stimulating neural activity, allowing for high-resolution interfacing with neural tissue.
  • Virus constructs: Genetically engineered viruses used for delivering genes into neurons, enabling the study of gene function or the manipulation of neural activity.
  • Wire electrodes: Thin metal wires used for recording electrical signals from or delivering electrical stimulation to neurons, essential for electrophysiological studies.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.