Consumables

pAAV-hsyn-GRAB_DA2hVirus constructPI: Li. Activity: Constitutive-
MFC_400/430-0.48_2.6mm_MF2.5_FLTOptic fiber designOptic Fiber Glass: Core diameter: 400um - Outer diameter: 430um - NA: 0.48 Length: 2.6mm Metal Ferule OD: 2.5mm Flat tip-
AAV9-hSyn-eCB2.1Virus constructTiter: ≥1*10E13vg/ml,50ul-
rAAV-PV-GCaMp6m-Flag-WPRE-hGH polyAVirus construct--
hCMV-dlox-SaCas9(rev)-dlox-bGHp(A)-hU6-sgRNA(mRosa26)Virus constructsingle-stranded genome-
HB980TOptic fiber designSingle Mode Polarization-Maintaining Fiber, 980 nm, Bow-Tie. The supplier provides a numerical aperture of 0.13 - 0.15.-
rAAV-EF1α-DIO-DTA-WPRE-hGH polyAVirus construct--
AAV9-EFS-DIO-CRFmutVirus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV8-hSyn-dTomatoVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
rAAV-EF1α-DIO-Kir2.1-P2A-EGFP-WPRE-hGH polyAVirus construct--
AAV-hThy1-eNpHR3.0-EYFP Virus constructThird-generation Optogenetic Inhibition: eNpHR3.0-
PRS2(8x)-hDBH-chI[4x:sh(NS)]-EGFP-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-CRH-GCaMP6s-WPRE-hGH polyAVirus construct--
AAV SYN flex PSAM4 GlyR IRES EGFPVirus constructCre requirement: Cre-dependent. PI: Sternson-
PFD-rAAV-hSyn-ArcLight-Q239-WPRE-hGH polyAVirus construct--
pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherryVirus constructActivity: Flp dependent. PI: Cepko-
hCMV-chI-EGFP_2A_iCre-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-dlox-ChroME2f_jGCaMP8m_rKv2.1(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
AAV9-hSyn-gACh3.8Virus constructTiter: ≥1*10E13vg/ml,50ul-
rAAV-nEF1a-fDIO-NpHR3.0-mCherry-WPRE-hGH polyAVirus construct--
AAV2-hSyn-CreVirus constructTiter: ≥5*10E12vg/ml. Volume: 50ul-
rAAV-CBh-EGFP-WPRE-hGH polyA-bGHpAVirus construct--
pAAV-Ef1a-DIO-PPO-VenusVirus constructActivity: Cre dependent. PI: Bruchas-
shortCAG-mLCK (N-term.)_StayGold-E138D-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hGFAP-chI[4x:sh(NS)]-HA_hM3D(Gq)-bGHp(A)Virus constructsingle-stranded genome-
hCMV-hHBbI/E-dlox-hALPP_GPI(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-EF1α-DIO-CRF1.0Virus construct--
rAAV-CaMKIIa-CRE-P2A-mCherry-WPRE-hGH polyAVirus construct--
pAAV-Ef1a-Con/Foff 2.0-oScarletVirus constructPI: Deisseroth. Activity: Cre dependent-
shortCAG-dlox-Peredox(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-CAG-fDIO-mNeonGreenVirus constructPI: Gradinaru. Activity: Flp dependent-
rAAV-TRE3G-DIO-hChR2(H134R)-EYFP-WPRE-hGHpAVirus construct--
pTight-HA_hM3D(Gq)-WPRE-bGHp(A)-rp(A)-pRAM-d2tTA-SV40p(A)Virus constructsingle-stranded genome-
pAAV-EF1a-iCreVVirus constructPI: Zeng-
AAV9-CaMKIIa-mCherryVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
L7-6-EGFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-EYFP_NaV1.2(II-III)-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-hSyn-CaMPARI2-WPRE-pAVirus construct--
AAV9-hSyn-NE2mmut(NE3.1mut)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
pAAV.CAG.GCaMP6s.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Constitutive-
hSyn1-chI-mCherry_2A_iCre-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hSyn1-chI-OxLight(ctr)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hEF1α-dlox-hMAPT(P301L)(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-EF1α-DIO-dLight1.1-WPRE-hGH polyAVirus construct--
hSyn1-ChroME2f_jGCaMP8m_rKv2.1-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-VGAT1-CRE-mCherry-WPRE-hGH polyAVirus construct--
hGFAP-chI-hERT2_iCre_hERT2(c.-d.)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-TRE3G-OChIEF-P2A-mCherry-WPRE-hGHpAVirus construct--
rAAV-CAG-fDIO-Arch-EYFP-WPRE-hGH polyAVirus construct--
AAV9-hSyn-DIO-mCherryVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
hEF1α/hTLV1-Coff/Fon(hM4D(Gi)_mCherry)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV-CAG-eYFPVirus constructPI: Gradinaru. Activity: Constitutive-
FP1000ERTOptic fiber design0.50 NA, Ø1000 µm Core Multimode Fiber, Low OH-
rAAV-hSyn-CRF3.0(1)-WPRE-hGH polyAVirus construct--
rAAV-EF1a-DIO-DA4.3-WPRE-hGH polyAVirus construct--
pAAV-hSyn-mCherryVirus constructPI: Deisseroth. Activity: Constitutive-
hGFAP-Voltron2-WPRE-hGHp(A)Virus constructsingle-stranded genome-
FG200AEAOptic fiber designØ200 µm Core, 0.22 NA, Solarization-Resistant Step-Index Multimode Fiber, Acrylate-Coated-
hSyn1-chI-rPSD-95_mTagBFP2-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomatoVirus constructPI: Ting. Activity: Cre dependent-
A8x32-Poly2-5mm-20s-lin-160Silicon probe designNeuroNexus 256ch, 8 shank, poly 2 with 20µm vertical pitch, 150µm shank spacing (A8x32-Poly2-5mm-20s-lin-160)-
hEF1α-dFRT-EBFP2_tTAn(rev)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hGFAP-EBFP2_iCre-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-hSyn-jGCamp7s-P2A-nls-dTomatoVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40Virus constructPI: Looger. Activity: Cre dependent-
rAAV-Cfos-EYFP-WPRE-hGHpAVirus construct--
rAAV-D2-CRE-WPRE-bGH polyAVirus construct--
hSyn1-dlox-mCyRFP1(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
FFM10026Optic fiber designØ100 µm Core, 0.26 NA, InF₃ Multimode Fiber-
hEF1α-C-CreintG-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-EF1α-DIO-K-CaMPARI-WPRE-hGH polyAVirus construct--
pAAV.Syn.NES-jRGECO1b.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Constitutive-
AAV9-hSyn-DIO-MT2.0Virus constructTiter: ≥1*10E13vg/ml,50ul-
pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPREVirus constructActivity: Constitutive. PI: Deisseroth-
pAAV-hSyn-DIO-mCherryVirus constructPI: Roth. Activity: Cre dependent-
PFD-rAAV-EF1a-DIO-R-iGluSnFR1-WPRE-hGH polyAVirus construct--
hCMV-chI-Cre-SV40p(A)Virus constructself-complementary genome-
AAV2-CAG-GFPVirus constructTiter: ≥5*10E12vg/ml Volume: 50ul-
rCRF-chI-EGFP_2A-WPRE-hGHp(A)Virus constructsingle-stranded genome-
Buzsaki A4x16_poly3_6mm20s_200_160Silicon probe designNeuroNexus 64ch, 4 shanks, poly 3, 200µm shank spacing (Buzsaki Poly 3 custom design a4x16_poly3_6mm20s_200_160)-
hVDM2-SV40I-EGFP-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-Pyronic-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pLenti CMV GFP Hygro (656-4)Virus construct3rd-gen lentiviral GFP expression vector. CMV promoter. Hygromycin resistance. PI: Campeau.-
pAAV-P3-Alb-mScarletVirus construct--
rAAV-hSyn-CRE-mCherry-WPRE-hGH polyAVirus construct--
hEF1α/hTLV1-Con/Fon(bReaChES_EYFP)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV-DJ-EF1a-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
rAAV-hSyn-Con/Fon-EYFP-WPRE-hGH polyAVirus construct--
AAV-synP-DIO-sTpEpB  (Addgene 52473)Virus constructMonosynaptic Tracing-
eHGT_410m-HBB-SYFP2-WPRE3-bGHp(A)Virus constructsingle-stranded genome-
AAV9-hSyn-GPR52Virus constructTiter: ≥1*10E13vg/ml,50ul-
YB300-6/125-PMOptic fiber designYtterbium-Doped SM PM Active Fiber, Core-Pumped, Single-Clad-
rAAV-hSyn-DIO-EYFP-WPRE-hGH polyAVirus construct--
rAAV-CMV-CRE-P2A-CRE-WPRE-bGH polyAVirus construct--
rAAV-mDLx-eNpHR3.0-EYFP-WPRE-hGH polyAVirus construct--
hSyn1-dlox-GCaMP6s_2A_NLS_dTomato(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV-EF1a-mCherry-IRES-WGA-Cre Virus constructTranssynaptic Tracers: WGA-Cre (AAV2, 5 & 8)-
YB1200-10/125DCOptic fiber designYtterbium-Doped LMA Double-Clad Fiber, 10 µm Core Diameter-
pAAV-nEF-Con/Fon-ChRmine-oScarletVirus constructActivity: Cre and Flp dependent. PI: Deisseroth-
hGFAP-chI[3x:sh(NS)]-Lck_GCaMP6f-SV40p(A)Virus constructsingle-stranded genome-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

  • Optic fiber designs: Thin, flexible fibers used for transmitting light signals, crucial for optical neural imaging and optogenetic manipulation of neurons.
  • Silicon probe designs: Microfabricated devices designed for recording or stimulating neural activity, allowing for high-resolution interfacing with neural tissue.
  • Virus constructs: Genetically engineered viruses used for delivering genes into neurons, enabling the study of gene function or the manipulation of neural activity.
  • Wire electrodes: Thin metal wires used for recording electrical signals from or delivering electrical stimulation to neurons, essential for electrophysiological studies.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.