Consumables

mDlx-HBB-chI-Con/Fon(EYFP)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hEF1α-dlox-jGCaMP7f(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV9-hsyn-HA2.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
hSyn1-ChRmine_MTS_EYFP_ERES-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hEF1α-dlox-myr_iRFP670_V5_2A_(post)eGRASP(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pGP-AAV-CAG-FLEX-jGCaMP8m-WPREVirus constructPI: GENIE. Activity: Cre-dependent1
AAV9-hsyn-DIO-SST2.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
AAAV9-hsyn-GABA0.8Virus constructTiter: ≥1*10E13vg/ml,50ul-
rAAV-CaMKIIa-DIO-oChIEF-P2A-dTomato-WPRE-bGH polyAVirus construct--
AAV2-U6-shRNA(scramble)-CMV-GFPVirus constructTiter: ≥5*10E12vg/ml Volume: 50ul-
shortCAG-iCre-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-TRE-tight-hM3D(Gq)-mCherry-WPRE-hGH polyAVirus construct--
rAAV-hSyn-EGFP-P2A-CRE-WPRE-hGH polyAVirus construct--
pAAV-hsyn-rDA3mVirus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV9-hSyn-DIO-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
hEF1α/hTLV1-dFRT-mSyp1_EGFP(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-EF1α-post-mGRASP-WPRE-hGH polyAVirus construct--
AAV-CAG-ChR2-GFPVirus constructNeural Activators-
rAAV-TRE3G-GFP-2A-TVA-WPRE-hGHpAVirus construct--
hDlx-HBB-chI-dlox-GtACR2_mCyRFP1(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP-WPRE-hGH polyAVirus construct--
hSyn1-chI-dlox-mNr3c2(ci)_V5(rev)-dlox-WPRE3-bGHp(A)Virus constructsingle-stranded genome-
shortCAG-mLCK (N-term.)_mTagBFP2-WPRE-SV40p(A)Virus constructsingle-stranded genome-
AAV6-CD68-Cre-P2A-GFPVirus constructTiter: ≥5*10E12vg/ml. Volume: 50ul-
hSyn1-chI-dlox-mito-mEos2(rev)-dlox-SV40p(A)Virus constructself-complementary genome-
rAAV-CAG-FLEX-ArchT-EGFP-WPRE-SV40 polyAVirus construct--
pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Cre dependent-
rAAV-hSyn-iGluSnFR(A184S)-P2A-mCherry-WPRE-hGH polyAVirus construct--
AAV9-U6-shRNA(scramble)-CMV-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
E2-16chSilicon probe design<p>Cambridge Neurotech E-series, 16ch, 1 shank, edge</p>-
AAV-EF1a-DIO-eNpHR3.0-EYFP Virus constructThird-generation Optogenetic Inhibition: eNpHR3.0-
hGFAP-hM4D(Gi)_mCherry-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40Virus constructPI: Looger. Activity: Cre dependent-
rAAV-hSyn-UDP0.4(UDP1.0)Virus construct--
hSyn1-chI-dlox-CaMPARI2(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
mDlx-HBB-chI-iCre-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-CAG-miRFP720-P2A-GFPVirus construct--
hEF1α-dlox-GCaMP6s_2A_NLS_dTomato(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-SfRE_rCaM_Oki-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV-CAG-FLPX-rc [ChrimsonR-tdTomato]Virus constructActivity: Flp dependent. PI: Boyden-
AAV2-CaMKIIa-DIO-hChR2(H134R)-mCherryVirus constructTiter: ≥5*10E12vg/ml. Volume: 50ul-
pAAV_hSyn-PdCO-mScarlet-WPREVirus constructActivity: Constitutive. PI: Yizhar-
AAV-CAG-FLEX-ArchT-tdTomatoVirus constructNeural Silencers (no AAV6)-
pAAV-hSyn-mScarletVirus constructPI: Deisseroth. Activity: Constitutive-
mCaMKIIα-EGFP-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV-CaMKIIa-C1V1(E162T)-TS-p2A-EYFP-WPREVirus constructCell Filling Variants-
pAAV-Ef1a-Con/Fon-mCherryVirus constructPI: Deisseroth. Activity: Cre and Flp dependent-
rAAV-EF1α-DIO-GCaMP5G-WPRE-hGH polyAVirus construct--
AAV9-hSyn-NPYmutVirus constructTiter: ≥1*10E13vg/ml,50ul-
AAV9-CaMKIIa-Cre-P2A-GFPVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
pAAV-hsyn-rDA2hVirus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV9-hSyn_Ado1.0-mScarlet(B10_mSca)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
hSyn1-chI-dFRT-Chronos_EGFP(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
mCaMKIIα-SwiChRca_MTS_EYFP-WPRE-hGHp(A)Virus constructsingle-stranded genome-
TRE3G-chI-myr_mScarlet-I_2A_(post)eGRASP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV8-CMV-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
AAV9-hsyn-DIO-eCB2.0mutVirus constructTiter: ≥1*10E13vg/ml, 50ul-
scAAV8-CMV-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
AAV9-hsyn-rDA1.2aVirus constructTiter: ≥1*10E13vg/ml,50ul-
rAAV-mOXT-EGFP-WPRE-hGH polyAVirus construct--
AAV9-CaMKII-GCaMP7b-X-NLSVirus constructTiter: ≥1*10E13vg/ml,50ul-
H1Silicon probe designCambridge Neurotech H1, 64ch, 2 shanks, high density sites, poly 4, 250µm shank spacing-
mCaMKIIα-NES_Twitch-GR-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-hSyn-NES-jRGECO1a-WPREVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
rAAV-hSyn-axon-GCaMP6m-WPRE-hGH polyAVirus construct--
AAV8-EF1a-DIO-GCaMP6m-P2A-nls-dTomatoVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
hSyn1-chI-dlox-eNpHR3.0_2A_jGCaMP8m(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-Tmem119-CRE-WPRE-hGH polyAVirus construct--
AAV9-hSyn-rACh1.4Virus constructGRAB Sensor1
hSyn1-ChrimsonR_LSSmOrange-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-dlox-SF_Venus_iGluSnFR(A184S)(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
CB4-chI-EGFP-SV40p(A)Virus constructsingle-stranded genome-
AAV-CaMKIIa-hChR2(E123T/T159C)-mCherryVirus constructSecond-generation Ultrafast Optogenetic Control-
rAAV-ETV1-2M-CRE-WPRE-hGH polyAVirus construct--
rAAV-EF1α-DIO-hChR2(H134R)-mCherry-WPRE-hGH polyAVirus construct--
c-Fos-chI-hERT2_iCre_hERT2(c.-d.)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-S5E2-GFP-fGFPVirus constructPI: Dimidschstein. Activity: Constitutive-
pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Cre dependent-
PMHN3NOptic fiber designPolarization-Maintaining Highly Nonlinear Fiber, -3 Normal Dispersion at 1550 nm-
rAAV-EF1α-DIO-lck-GCaMp6f -WPRE-hGH polyAVirus construct--
AAV9-CMV-CreVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
mCaMKIIα(short)-(pre)eGRASP(p32; cyan)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-AchLightR-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-CaMKIIa-QuasAr2-mOrange-WPRE-hGH polyAVirus construct--
P1-16chSilicon probe designCambridge Neurotech P-series, 16ch, 1 shank, poly 2-
hChAT-tdTomato-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pAAV-hSyn-DIO-ChrimsonR-mRuby2-STVirus constructActivity: Cre dependent. PI: Adesnik-
rAAV-hSyn-ChrimsonR-EGFP-WPRE-hGH polyAVirus construct--
rAAV-hSyn-ATP1.0_mutVirus construct--
pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrineVirus constructCre requirement: none. PI: Roth-
pAAV-hSyn-GRAB_ACh3.0Virus constructPI: Li. Activity: Constitutive-
AAV9-GFAP-hM4D(Gi)-mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
hSyn1-ATeam1.03YEMK-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-hHBbI/E-TVA950_mCherry_2A_oG-hGHp(A)Virus constructsingle-stranded genome-
AAV9-hsyn-5HT3.5Virus constructTiter: ≥1*10E13vg/ml, 50ul-
hGFAP-GeNL-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-dlox-GACh2.0(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
PMHN1Optic fiber designPolarization-Maintaining Highly Nonlinear Fiber, +1 Anomalous Dispersion at 1550 nm-
rAAV-CAG-hChR2(H134R)-tdTomato-WPRE-SV40 polyAVirus construct--
shortCAG-chI[4x:sh(NS)]-mScarlet-I-WPRE-bGHp(A)Virus constructsingle-stranded genome-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

  • Optic fiber designs: Thin, flexible fibers used for transmitting light signals, crucial for optical neural imaging and optogenetic manipulation of neurons.
  • Silicon probe designs: Microfabricated devices designed for recording or stimulating neural activity, allowing for high-resolution interfacing with neural tissue.
  • Virus constructs: Genetically engineered viruses used for delivering genes into neurons, enabling the study of gene function or the manipulation of neural activity.
  • Wire electrodes: Thin metal wires used for recording electrical signals from or delivering electrical stimulation to neurons, essential for electrophysiological studies.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.