Consumables

hEF1α/hTLV1-dFRT-DTR_2A_mCherry(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-EF1a-DIO-hChR2(C128S/D156A)-mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
FG200LEPOptic fiber designØ200 µm Core Polyimide-Coated Multimode Fibers-
AAV9-hsyn-HA1.0mut (His1.0mut)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
rAAV-TRE3G-Cre-WPRE-hGHpAVirus construct--
rCRF-chI-iCre-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-iGluSnFR3.v857.GPI(c.-o.)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
GF1BOptic fiber designLow Loss Photosensitive Fiber, 1500 - 1600 nm, 10.4 µm MFD, 0.13 NA-
shortCAG-SF_iGluSnFR(A184S)-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hSyn1-ConVERGD(TVA950_mCherry)-WPRE3-SV40p(A)Virus constructsingle-stranded genome-
rAAV-D2(ENK)-CRE-WPRE-bGH polyAVirus construct--
pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrineVirus constructCre requirement: Cre-dependent. PI: Roth-
hSyn1-dlox-TVA_2A_mCherry_2A_oG(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV1-hSyn-CreVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
pAAV-ihSyn1-DIO-tTAVirus constructExpression System: Cre-dependent and inducible Synapsin promoter (ihSyn). Activity: Cre-dependent expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified tTA expression.. PI: Gradinaru-
hSyn1-chI-GLPLight(ctr)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hEF1α/hTLV1-dlox-EGFP(rev)-dlox-SV40p(A)Virus constructself-complementary genome-
AAV9-EF1a-mRuby3-S1P1.0Virus constructTiter: ≥1*10E13vg/ml,50ul-
AAV-Syn-H2B-jGCaMP8s-WPREVirus constructPI: Looger. Activity: Constitutive-
pAAV-hSyn-GRAB_DA1mVirus constructPI: Li. Activity: Constitutive-
rAAV-CaMKIIa-NpHR3.0-EYFP-WPRE-hGH polyAVirus construct--
Flexible_Polyimide_64ch_poly3Silicon probe designPolyimide probe-
rAAV-nEF1α-FDIO-TVA-tdTomato-WPRE-hGH polyAVirus construct--
hCMV-chI-ECFP-SV40p(A)Virus constructself-complementary genome-
AAV9-TRE-5-HT1.0(5-HT2.1)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
hSyn1-chI-dlox-stGtACR2_mCerulean(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
shortCAG-dlox-mScarlet-I_2A_HA_hM3D(Gq)(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-dlox-GCaMP6f(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hCMV-chI-EBFP2-SV40p(A)Virus constructself-complementary genome-
hCMV-chI-FLIP(c.-o.)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV-CamKII-Ado1.0Virus construct--
AAV-EF1a-DIO-hChR2(E123T/T159C)-p2A-EYFP-WPREVirus constructCell Filling Variants-
AAV-EF1a-DIO-hChR2(E123T/T159C)-EYFPVirus constructSecond-generation Ultrafast Optogenetic Control-
rAAV-hSyn-ACh2.0-WPRE-PAVirus construct--
pAAV.Syn.Flex.GCaMP6m.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Cre dependent-
pAAV-mDlx-GCaMP6f-Fishell-2Virus constructPI: Fishell. Activity: Constitutive-
mCaMKIIα(short)-ePdCO_EGFP-WPRE-synp(A)Virus constructsingle-stranded genome-
ER16-8/125Optic fiber designErbium-Doped LMA Active Fiber, 16.0 dB/m Peak Core Absorption at 1530 nm-
hSyn1-chI-tdTomato-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-cfos-CreERT2-Flag-WPRE-hGH polyAVirus construct--
hSyn1-chI-EGFP_hRAB5A-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pAAV-CAG-DIO-ChR2(H134R)-eYFPVirus constructActivity: Cre dependent. PI: Gradinaru-
pAAV-Ef1a-Con/Foff 2.0-GCaMP6MVirus constructPI: Deisseroth. Activity: Cre dependent-
rAAV-CaMKIIa-DIO-GCaMp6s-WPRE-hGH polyAVirus construct--
scAAV1-CMV-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
AAV-hSyn-Coff/Fon hChR2(H134R)-EYFP-WPREVirus constructINTRSECT (AAV5 & DJ)-
pAAV-Ef1a-sRGECOVirus constructPI: Deisseroth. Activity: Constitutive-
hEF1α-EGFP_2A_FLPo-WPRE-bGHp(A)Virus constructsingle-stranded genome-
P3-mAlb_Dendra2-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-TBG -Luciferase-bGHpAVirus construct--
pAAV-GFAP-hM3D(Gq)-mCherryVirus constructCre requirement: none. PI: Roth-
hDlx-HBB-chI-dlox-hM4D(Gi)_EGFP(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-EF1α-fDIO-taCasp3-EGFP-WPRE-hGH polyAVirus construct--
GIF50EOptic fiber designØ50 µm Core Graded-Index Multimode Optical Fiber, 0.20 NA, OM4-
pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPREVirus constructActivity: Constitutive. PI: Yizhar-
hEF1α-dlox-eArch3.0_EYFP(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
mDlx-HBB-chI-dlox-hChR2(H134R)_mCherry(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV-EF1a-jGCaMP8f-WPREVirus constructPI: Looger. Activity: Constitutive-
rAAV-D2-fDIO-CRE-mCherry-WPRE-hGH polyAVirus construct--
pAAV-double floxed-eNpHR-EYFP-WPRE-pAVirus constructActivity: Cre dependent. PI: Deisseroth-
rAAV-hSyn-DIO-mGFP-T2A-Synaptophysin-mRuby-WPRE-hGH polyAVirus construct--
AAV-EF1a-DIO-mCherryVirus constructControl Fluorophores-
pAAV-hSynapsin-FLEX-soCoChR-GFPVirus constructActivity: Cre dependent. PI: Boyden-
pGP-AAV-GFAP-iGABASnFR2-WPREVirus constructPI: GENIE. Activity: Constitutive-
hSyn1-M13_TEV(C-term.)_2A_dTomato(non-c.d.)-WPRE-bGHp(A)-SV40p(A)Virus constructsingle-stranded genome-
rAAV-hSyn-hChR2(H134R)-mCherry-WPRE-hGH polyAVirus construct--
pAAV-hsyn-rDA3hVirus constructTiter: ≥1*10E13vg/ml, 50ul-
hSyn1-dlox-rM3D(Gs)_mCherry(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-D2-mCherry-WPRE-bGH polyAVirus construct--
hSyn1-EGFP_iCre-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-HA_hKORD_IRES_mCitrine-WPRE-bGHp(A)Virus constructsingle-stranded genome-
mPer2-dlox-ELuc_PEST(rev)-dlox-hGHp(A)Virus constructsingle-stranded genome-
CAG-EGFP_Cre-WPRE-SV40p(A)Virus constructsingle-stranded genome-
AAV9-hsyn-DIO-eCB2.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
rAAV-CaMKIIa-FLP-EGFP-WPRE-hGH polyAVirus construct--
pAAV.TBG.PI.Null.bGHVirus constructPI: Wilson. Activity: Constitutive-
AAV6-U6-shRNA(scramble)-CMV-GFPVirus constructTiter: ≥5*10E12vg/ml Volume: 50ul-
AAV2 retro-hSyn-GCaMP6f-P2A-nls-dTomatoVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
rAAV-GFAP-3XFlag-WPRE-hGH polyAVirus construct--
AAV1-ICAM2-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
Virus 1Virus construct--
AAV2-SM22a-GFPVirus constructTiter: ≥5*10E12vg/ml Volume: 50ul-
rAAV-CaMKIIa-GCaMP6s-WPRE-hGH polyAVirus construct--
AAV7-CMV-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
shortCAG-tdTomato-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pAAV.hSyn.iGluSnFR3.v857.PDGFRVirus constructPI: Podgorski. Activity: Constitutive-
UM22-100 (Ø 100/110µm, 0.22 NA)Optic fiber designØ100 µm Core Solarization-Resistant, Polyimide-Coated Multimode Fiber-
pAAV.GFAP.eGFP.WPRE.hGHVirus constructPI: Wilson. Activity: Constitutive-
hSyn1-FRT-dlox-hChR2(H134R)_EYFP(rev)-dlox-FRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-EF1α-DIO-ReachR-citrine-WPRE-hGH polyAVirus construct--
AAV-CaMKIIa-C1V1(t/t)-TS-p2A-EYFP-WPREVirus constructCell Filling Variants-
hSyn1-chI-dLight1.3b-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV-DJ-CMV-RFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
rAAV-CaMKIIa-mito-cpYFP-WPRE-hGH polyAVirus construct--
pAAV.CAG.iAChSnFRVirus constructPI: Looger. Activity: Constitutive-
AAV9-CaMKIIα-eCB2.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV9-TRE3G-hM3D(Gq)-mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
rAAV-hSyn-CaMPARI-WPRE-hGH polyAVirus construct--
AAV9-hSyn-5-HT1.0(5-HT2.1)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-PuroVirus constructExpresses SARS-CoV-2 M protein. Puromycin resistance. EF1a promoter. PI: Krogan.-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

  • Optic fiber designs: Thin, flexible fibers used for transmitting light signals, crucial for optical neural imaging and optogenetic manipulation of neurons.
  • Silicon probe designs: Microfabricated devices designed for recording or stimulating neural activity, allowing for high-resolution interfacing with neural tissue.
  • Virus constructs: Genetically engineered viruses used for delivering genes into neurons, enabling the study of gene function or the manipulation of neural activity.
  • Wire electrodes: Thin metal wires used for recording electrical signals from or delivering electrical stimulation to neurons, essential for electrophysiological studies.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.