Consumables

AAV9-CaMKIIa-DIO-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
pAAV.hSyn-FLEX.iGABASnFRVirus constructPI: Looger. Activity: Cre dependent-
hSyn1-ConVERGD(EGFP)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-hsyn-SST2.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
shortCAG-mCherry_2A_hM4D(Gi)_HA(2x)_rNrxn1-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pAAV-hSyn-GRAB-gDA3mVirus constructPI: Li. Activity: Constitutive-
hSyn1-GRAB(NE2h)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV-hsyn-OxLightVirus construct--
AAV9-CamkIIa-GCAMP6fVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
pAAV-hSyn-GRAB_NE1hVirus constructPI: Li. Activity: Constitutive-
rAAV-GFAP-EGFP-WPRE-hGH polyAVirus construct--
FT600UMTOptic fiber designØ600 µm Core, 0.39 NA, High OH TECS-Clad Multimode Fiber-
mCaMKIIα(short)-lox71-(pre)eGRASP(p32; cyan)_2A_3xNLS_mScarlet3-lox66-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-hSyn-hChR2(H134R)-EYFP-WPRE-hGH polyAVirus construct--
mDlx-HBB-chI-dlox-EGFP(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-TH-3XFlag-WPRE-hGH polyAVirus construct--
c-Fos-c-FosI-rtTA3G-hGHp(A)Virus constructsingle-stranded genome-
hGFAP-Cre-4x6(miRT)-hGHp(A)Virus constructsingle-stranded genome-
AAV5-CMV-GFPVirus constructTiter: ≥5*10E12vg/ml Volume: 50ul-
rAAV-EF1α-DIO-GRAB-NE1m-WPRE-hGH polyAVirus construct--
rAAV-TH-EYFP-WPRE-pAVirus construct--
shortCAG-dlox-ReaChR_mCitrine(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-hSyn-EGFP-2A-TVA-2A-RVG-WPREsVirus construct--
rAAV-TRE-tight-hM4D(Gi)-mCherry-WPRE-hGHpAVirus construct--
rAAV-D1-EGFP-WPRE-hGH polyAVirus construct--
rAAV-hSyn-fDIO-GCaMp6f-WPRE-hGH polyAVirus construct--
hGFAP-tdTomato-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-hHBbI/E-TVA950_mCherry_2A_ooG-hGHp(A)Virus constructsingle-stranded genome-
AAV-hSyn1-SIO-eOPN3-mScarlet-WPREVirus constructActivity: Cre dependent. PI: Yizhar-
pAAV.Syn.GCaMP6s.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Constitutive-
pTight-mTagBFP2-WPRE-bGHp(A)-rp(A)-pRAM-d2tTA-SV40p(A)Virus constructsingle-stranded genome-
rAAV-GFAP-GCaMp6s-WPRE-hGH polyAVirus construct--
pAAV.CAG.Flex.GCaMP6f.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Cre dependent-
shortCAG-dlox-jGCaMP8s(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-D1-EGFP-WPRE-bGH polyAVirus construct--
AAV9-hsyn-Halo-AVP0.4Virus constructTiter: ≥1*10E13vg/ml,50ul-
hSyn1-GRAB(eCB2.0)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hEF1α-Con/Fon(oScarlet)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
Morris CellTag Barcode Library Version 1Virus construct19,973 barcodes for single-cell clonal analysis. Expresses EGFP. PI: Morris.-
AAV9-hsyn-mRuby3-NE2hVirus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV9-hSyn-MT1.0Virus constructTiter: ≥1*10E13vg/ml,50ul-
pAAV-hDlx-GiDREADD-dTomato-Fishell-5Virus constructCre requirement: none. PI: Fishell-
pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPREVirus constructPI: Kim. Activity: Cre-
pAAV.CAG.Flex.GCaMP6s.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Cre dependent-
CBh-EGFP-WPRE3-bGHp(A)Virus constructsingle-stranded genome-
rAAV-ChAT-CRE-P2A-EGFP-WPRE-hGH polyAVirus construct--
hSyn1-chI-dlox-mKate2(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pZac2.1-GfaABC1D-lck-jGCaMP8fVirus constructPI: Looger. Activity: Constitutive-
hSyn1-chI-dFRT-iGluSnFR3.v857.GPI(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
mCaMKIIα-dlox-EBFP2(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV.CAG.Flex.iGluSnFr.WPRE.SV40Virus constructPI: Looger. Activity: Cre dependent-
rAAV-hSyn-GRAB_eCB2.0-WPRE-hGH polyAVirus construct--
980HPOptic fiber designSingle Mode Fiber, 980 - 1600 nm, Ø125 µm Cladding-
rAAV-CaMKIIa-GCaMp6f-WPRE-hGH polyAVirus construct--
pAAV.Syn.Flex.GCaMP6s.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Cre-dependent-
EMG wireSingle wire electrode--
FG010LDAOptic fiber design(Ø10 µm Core, 0.10 NA) Ø10 µm Core, 0.10 NA, Step-Index Multimode Optical Fiber. The supplier provides a numerical aperture of 0.100 - 0.115.-
AAV-CamKIIa-jGCaMP8s-WPREVirus constructPI: Looger. Activity: Constitutive-
rAAV-EF1α-DIO-Tau40-Flag-WPRE-hGH polyAVirus construct--
rAAV-CaMKIIa-CRE-WPRE-hGH polyAVirus construct--
L7-6-tdTomato_2A_iCre-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV9-TRE-NE1m(NE2.1)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
mCaMKIIα-stGtACR2_mCerulean-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-mRuby2_2A_GCaMP6m-WPRE-SV40p(A)Virus constructsingle-stranded genome-
FFH20026Optic fiber designØ200 µm Core, 0.26 NA, Double-Clad InF₃ Multimode Fiber-
rAAV-SST-EGFP-bGH polyAVirus construct--
shortCAG-H2BJ_EGFP-WPRE-SV40p(A)Virus constructsingle-stranded genome-
mCaMKIIα(short)-hChR2(E123T/H134R/T159C)_mTurquoise2_rKv2.1-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-mCD68-CRE-WPRE-PAVirus construct--
rAAV-TRPV1-CRE-WPRE-hGH polyAVirus construct--
hEF1α-dFRT-EGFP_2A_iCre(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFPVirus constructActivity: Cre and Flp dependent. PI: Deisseroth-
AAV9-EFS-DIO-ATP1.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
shortCAG-dlox-iRFP670(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-Ki67-EYFP-WPRE-hGH polyAVirus construct--
AAV5-GfaABC1D-Ado1.1mVirus constructTiter: ≥1*10E13vg/ml,50ul-
rAAV-mTH-GCaMp6m-WPREsVirus construct--
AAV9-hSyn-GRAB-rNTS0.5Virus constructTiter: ≥1*10E13vg/ml, 50ul-
FG365UECOptic fiber designØ365 µm Core, 0.22 NA, High OH TECS Double-Clad Multimode Fiber-
pAAV-FLEX-EGFPL10aVirus constructExpression System: EF1a-driven and Cre-dependent. Activity: EGFP-tagged ribosomal L10a. PI: Heintz, Nectow, Schmidt-
FT600EMTOptic fiber designØ600 µm Core, 0.39 NA, Low OH TECS-Clad Multimode Fiber-
FG550LECOptic fiber designØ550 µm Core, 0.22 NA, Low OH TECS Double-Clad Multimode Fiber-
AAV-EF1a-DIO-ChR2(T159C)-EYFPVirus constructSecond-generation Ultrafast Optogenetic Control-
L7-6-EGFP_2A_iCre-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-GFaABC1D-EYFP-WPRE-SV40 polyAVirus construct--
rAAV-hSyn-fDIO-CRE-WPRE-hGH polyAVirus construct--
rAAV-EF1α-DIO-Jaws-EGFP-ER2-WPRE-hGH polyAVirus construct--
AAV-EF1a-jGCaMP8m-WPREVirus constructPI: Looger. Activity: Constitutive-
pAAV-Ef1a-fDIO-GCaMP6sVirus constructPI: Larsen. Activity: Flp dependent-
pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pAVirus constructPI: Bonhoeffer , Huebener , Rose. Activity: Cre dependent-
AAV-CaMKII-GFP (AAV2, 5 & 8)Virus constructControl Vectors-
rAAV-EF1α-DIO-NpHR3.0-mCherry-WPRE-hGH polyAVirus construct--
AAV5-hSyn-hChR2(H134R)-EYFP Virus constructOptical Excitation: ChR4-
hEF1α-dlox-nLightG(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-Con/Fon(hChR2(H134R)_EYFP)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV-CAG-FLEX-ArchT-GFPVirus constructNeural Silencers-
pAAV-CaMKIIa-EGFPVirus constructPI: Roth. Activity: Constitutive-
mCaMKIIα(short)-paCaMKII-WPRE3-synp(A)-SV40p(A)Virus constructsingle-stranded genome-
rAAV-hSyn-CRE-P2A-EGFP-WPRE-hGH polyAVirus construct--
pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pAVirus constructPI: Bonhoeffer , Huebener , Rose. Activity: Cre dependent-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

  • Optic fiber designs: Thin, flexible fibers used for transmitting light signals, crucial for optical neural imaging and optogenetic manipulation of neurons.
  • Silicon probe designs: Microfabricated devices designed for recording or stimulating neural activity, allowing for high-resolution interfacing with neural tissue.
  • Virus constructs: Genetically engineered viruses used for delivering genes into neurons, enabling the study of gene function or the manipulation of neural activity.
  • Wire electrodes: Thin metal wires used for recording electrical signals from or delivering electrical stimulation to neurons, essential for electrophysiological studies.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.