Consumables

rAAV-CMV-βGlobin-CRE-3XFlag-WPRE-hGH polyAVirus construct--
hSyn1-GRAB(gDA3h)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-hSyn-ChrimsonR-tdTomato-WPRE-bGH polyAVirus construct--
rAAV-EF1α-DIO-FLP-P2A-FLP-WPRE-hGH polyAVirus construct--
hSyn1-chI-Peredox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV-EF1a-DIO-SwiChRCA-TS-EYFP-WPREVirus constructSwiChRCA  (AAV2, 5, DJ)1
SHB1250Optic fiber designSingle Mode Spun Polarization-Maintaining Fiber, 1310 nm, Ø125 µm Cladding. The supplier provides a numerical aperture of 0.13 - 0.17.-
mCaMKIIα-GCaMP6m-WPRE-SV40p(A)Virus constructsingle-stranded genome-
PM2000Optic fiber designSingle Mode Polarization-Maintaining Fiber, 1850 - 2200 nm, PANDA-
hSyn1-CoChR(K237A)_GCaMP6f_Kv2.1-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-dlox-mMars(L274G)(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-TRE3G-DIO-EGFP-2A-TVA-WPRE-hGH polyAVirus construct--
AAV-hSyn-Coff/Fon EYFP-WPREVirus constructINTRSECT (AAV5 & DJ)-
rAAV-Ef1α-DIO-BFP-2A-TK-WPRE-hGH polyAVirus construct--
rAAV-EF1α-DIO-ACh2.0-WPRE-PAVirus construct--
AAV2 retro-hSyn-hM4D(Gi)-mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
rAAV-EF1α-DIO-hChR2(H134R)-EYFP-WPRE-hGH polyAVirus construct--
Human sgRNA library Brunello in lentiGuide-PuroVirus constructHuman sgRNA library targeting 19,114 genes with 76,441 sgRNAs + 1000 controls. Puromycin selection. PI: Doench, Root.-
hCMV-chI-EGFP-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hEF1α/hTLV1-dFRT-eOPN3_mScarlet(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-dlox-hChR2(H134R)_EYFP(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-TH-EYFP-WPRE-hGH polyAVirus construct--
pAV-EF1a-DIO-mRuby3-PGE2-1.0Virus constructTiter: ≥1*10E13vg/ml,50ul-
FG910LECOptic fiber designØ910 µm Core, 0.22 NA, Low OH TECS Double-Clad Multimode Fiber-
AAV5-GFaABC1D-DA3hVirus constructTiter: ≥1*10E13vg/ml,50ul-
PS1060Optic fiber designPhotosensitive Select Cut-Off Fiber, 980 - 1060 nm, 6.2 µm MFD, 0.13 NA-
FG1000UEAOptic fiber designØ1000 µm Core Glass Clad Silica Multimode Optical Fiber, 0.22 NA-
pGP-AAV-CAG-FLEX-jGCaMP7f-WPREVirus constructPI: Kim. Activity: Cre dependent-
AAV9-hsyn-r5HT1.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
rAAV-EF1α-DIO-VSVG-WPRE-hGH polyAVirus construct--
pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-PuroVirus constructExpresses SARS-CoV-2 E protein. Puromycin resistance. EF1a promoter. PI: Krogan.-
pAAV-TREtight-mTagBFP2-B19GVirus constructPI: Wickersham.-
mCaMKIIα-CoChR(K237A)_GCaMP6f_Kv2.1-WPRE-bGHp(A)Virus constructsingle-stranded genome-
mCaMKIIα-GCaMP6f-WPRE-SV40p(A)Virus constructsingle-stranded genome-
AAV9-hSyn-NES-jRCaMP1a-WPREVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
hEF1α-Coff/Fon(GCaMP6f)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-HisLightG-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-Ef1a-Con/Foff 2.0-GCaMP6FVirus constructPI: Deisseroth. Activity: Cre dependent-
rAAV-mCD68-EYFP-WPRE-PAVirus construct--
AAV DJ-hsyn-ATP1.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
CAG-dFRT-EGFP(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV9-CaMKIIa-ochIEF-tdTomatoVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
hSyn1-chI-RdLight1-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hGFAP-mTagBFP2_NLS-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-CAG-EYFP-WPRE-hGH polyAVirus construct--
FG200LEP (Ø 200/220µm, 0.22 NA)Optic fiber designØ200 µm Core Polyimide-Coated Multimode Fibers-
pAAV-hSyn-hChR2(H134R)-EYFPVirus constructActivity: Constitutive. PI: Deisseroth-
hSyn1-dFRT-hM3D(Gq)_mCherry(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
PM630-HPOptic fiber designSingle Mode Polarization-Maintaining Fiber, 620 - 850 nm, PANDA-
hEF1α-dlox-EGFP(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-EF1α-DIO-hM4D(Gi)-EGFP-WPREsVirus construct--
PM-405Optic fiber designSingle Mode Polarization-Maintaining Fiber, 400 - 488 nm, PANDA-
mCaMKIIα(short)-TVA950_V5_2A_G-WPRE3-synp(A)-SV40p(A)Virus constructsingle-stranded genome-
rAAV-EF1α-DIO-GCaMp6f-P2A-NLS-tdTomato-WPRE-hGH polyAVirus construct--
A4x16-5mm-50-500-703Silicon probe designSpecial Order-
AAV9-hSyn-Cyto-Ado2mVirus constructTiter: ≥1*10E13vg/ml,50ul-
AAV9-TRE-jRGECO1-P2A-NE2.1Virus constructTiter: ≥1*10E13vg/ml, 50ul-
ER80-8/125Optic fiber designErbium-Doped LMA Active Fiber, 80.0 dB/m Peak Core Absorption at 1530 nm-
hEF1α-dFRT-jGCaMP7s(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
mCaMKIIα-loxP-iRFP_2A_FLPo-loxP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
H2Silicon probe designCambridge Neurotech H2, 64ch, 2 shanks, linear, 25µm spacing, 250µm shank spacing-
rAAV-hSyn-NEmutVirus construct--
hEF1α-dlox-GAP43_GCaMP6f(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-hSyn-mCherry-WPRE-hGH polyAVirus construct--
hSyn1-chI-dFRT-GtACR2_EYFP(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pAVirus constructPI: Bonhoeffer , Huebener , Rose. Activity: Constitutive-
mCaMKIIα-ChRmine_MTS_mScarlet_Kv2.1_ERES-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-ChrimsonR_EGFP-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hGFAP-dlox-EBFP2_NLS(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pLenti-SV40-Puro-EF1a-mCherryVirus constructTiter: 1x10^8 IU/mL-
AAV-EF1a-fDIO-hChR2(H134R)-EYFP-WPREVirus constructINTRSECT (AAV5 & DJ)-
AAV-PHP.EB-hSyn-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
rAAV-VGAT1-hM3D(Gq)-mCherry-WPRE-hGH polyAVirus construct--
pAAV-Ef1a-fDIO-JRGECO1aVirus constructPI: Jensen. Activity: Flp dependent-
rAAV-nEf1α-FDIO-EGFP-2A-TVA-WPRE-hGH polyAVirus construct--
hEF1α-dFRT-hChR2(E123T/T159C)_EGFP(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
FG400UEP Optic fiber designØ400 µm Core Polyimide-Coated Multimode Fibers-
hSyn1-chI-PAClight1(P78A-ctrl)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
A4x2-tet-5mm-150-200-121Silicon probe designtetrode to tetrode distance: 150µm-
hEF1α-dlox-ChRmine_MTS_mScarlet_ERES(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
Neuropixels 1.0 DummySilicon probe design960 channels in total to select from1
hCMV-dlox-SaCas9(rev)-dlox-bGHp(A)-hU6-sgRNA(control)Virus constructsingle-stranded genome-
P-25/250DC-PMOptic fiber designPassive LMA PM Fiber, Matched to YB1200-25/250DC-PM-
pAAV-S5E2-ChR2-mCherryVirus constructActivity: Constitutive. PI: Dimidschstein-
AAV9-GfaABC1D-ATP1.0mutVirus constructTiter: ≥1*10E13vg/ml, 50ul-
rAAV-hSyn-Flex-EGFP-T2A-Synaptophysin-mRuby-WPRE-hGH polyAVirus construct--
rAAV-EF1α-pre-mGRASP-2A-H2B-BFP-WPRE-hGH polyAVirus construct--
pAAV-hDlx-GqDREADD-dTomato-Fishell-4Virus constructCre requirement: none. PI: Fishell-
hEF1α/hTLV1-Con/Fon(Arch3.0_2A_EYFP)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-EF1α-DIO-K-CaMPARI(W391F V398L)-WPRE-hGH polyAVirus construct--
AAV9-CaMKIIa-hM3D(Gq)-mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
hSyn1-chI-AdoLightG-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-VGAT1-hChR2(H134R)-mCherry-WPRE-hGH polyAVirus construct--
pTight-HA_hM4D(Gi)-WPRE-bGHp(A)-pRAM-d2tTA-SV40p(A)Virus constructsingle-stranded genome-
mCaMKIIα-SomArchon_EGFP-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hCMV-dlox-SaCas9(rev)-dlox-bGHp(A)-hU6-sgRNA(Slc6a3)Virus constructsingle-stranded genome-
rAAV-hSyn-Jaws-EGFP-ER2-WPRE-pAVirus construct--
pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40Virus constructPI: Schreiter. Activity: Constitutive-
shortCAG-FLPo-WPRE-SV40p(A)Virus constructsingle-stranded genome-
FFM05026Optic fiber designØ50 µm Core, 0.26 NA, InF₃ Multimode Fiber-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

  • Optic fiber designs: Thin, flexible fibers used for transmitting light signals, crucial for optical neural imaging and optogenetic manipulation of neurons.
  • Silicon probe designs: Microfabricated devices designed for recording or stimulating neural activity, allowing for high-resolution interfacing with neural tissue.
  • Virus constructs: Genetically engineered viruses used for delivering genes into neurons, enabling the study of gene function or the manipulation of neural activity.
  • Wire electrodes: Thin metal wires used for recording electrical signals from or delivering electrical stimulation to neurons, essential for electrophysiological studies.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.