Consumables

AAV-GFAP104-ChR2-mCherryVirus constructGFAP Vectors (AAV8)-
AAV-CAG-jGCaMP8s-WPREVirus constructPI: Looger. Activity: Constitutive-
shortCAG-H2BJ_mScarlet-I-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pAAV-Ef1a-Con/Fon-BFPVirus constructPI: Deisseroth. Activity: Cre and Flp dependent-
rAAV-nEF1α-fDIO-hM4D(Gi)-EGFP-WPRE-hGH polyAVirus construct--
shortCAG-dlox-oG(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hGFAP-hHBbI/E-ATeam1.03-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-TRE3G-DIO-hM4D(Gi)-mCherry -WPRE-hGHpAVirus construct--
AAV-DJ-CMV-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
rAAV-D1-hChR2(H134R)-mCherry-WPRE-hGH polyAVirus construct--
AAV9-hSyn-DIO-eCB1.0Virus constructTiter: ≥1*10E13vg/ml,50ul-
pAAV-hsyn-Rdlight1Virus construct--
780HPOptic fiber designSingle Mode Fiber, 780 - 970 nm, Ø125 µm Cladding-
pAAV-Ef1a-Con/Foff 2.0-BFPVirus constructPI: Deisseroth. Activity: Cre dependent-
AAV2-CaMKIIa-CreVirus constructTiter: ≥5*10E12vg/ml. Volume: 50ul-
AAV-hSyn-eNpHR3.0-EYFP Virus constructThird-generation Optogenetic Inhibition: eNpHR3.0-
rAAV-EF1α-DIO-hChR2(E123T/T159C)-mCherry-WPRE-hGH polyAVirus construct--
AAV2 retro-hSyn-Flp-P2A-mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
A8x16-Poly2-5mm-20s-lin-160Silicon probe design*This probe has an non-uniform layout. See company catalog for more information: 2018ProbeCatalog_20180515_Web, p.67 Each shank has two sites in a linear layout, separated by 100µm, above the staggered tip. The sites are 100µm above the tip in the first shank, 200µm above the tip in the second shank, 300µm above the tip in the third, and so forth.-
L7-6-jGCaMP8m-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV-EF1a-DIO-eNpHR3.0-mCherryVirus constructThird-generation Optogenetic Inhibition: eNpHR3.0-
rAAV-CMV-DIO-NpHR3.0(V5)-F2A-ChR2-HA-hGH polyAVirus construct--
rAAV-CAG-DIO-hM3D(Gq)-mCherry-WPRE-hGH polyAVirus construct--
AAV9-hsyn-DIO-rDA1.2aVirus constructTiter: ≥1*10E13vg/ml,50ul-
pAAV-nEF-Con/Foff 2.0-NpHR3.3-EYFPVirus constructActivity: Cre dependent. PI: Deisseroth-
rAAV-mDlx-taCasp3-T2A-TEVp-WPRE-hGH polyAVirus construct--
AAV9-hsyn-NE2m(NE3.1)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-STVirus constructActivity: Cre dependent. PI: Adesnik-
hSyn1-chI[4x:sh(NS)]-EGFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-VGAT1-taCasp3-T2A-TEVp-WPRE-hGH polyAVirus construct--
pTight-EGFP-WPRE-bGHp(A)-pRAM-d2tTA-SV40p(A)Virus constructsingle-stranded genome-
rAAV-CMV-CRE-WPRE-hGH polyAVirus construct--
rAAV-TBG-CRE-WPRE-hGH polyAVirus construct--
AAV9-hSyn-5-HTmut(5-HT2.1-mut)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV-hSyn-Soma-jGCaMP8sVirus constructPI: Fyhn. Activity: Constitutive-
Buzsaki64LSilicon probe designNeuroNexus 64ch, 8 shanks, staggered, buzsaki-style, 200µm shank spacing, 10mm shanks (Buzsaki64L)-
hGFAP-TVA_2A_EGFP_2A_oG-WPRE-bGH(A)Virus constructsingle-stranded genome-
rAAV-CS-CRM4-αMHC-CRE-WPRE-hGH polyAVirus construct--
hEF1α-H2B_mTFP1-WPRE-hGHp(A)Virus constructsingle-stranded genome-
mCaMKIIα-hM4D(Gi)_EGFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pENN.AAV.CamKII0.4.eGFP.WPRE.rBGVirus constructPI: Wilson. Activity: Constitutive-
rAAV-CAG::FLEX-rev:: hM4D-2a-GFPVirus constructCre requirement: Cre-dependent. PI: Sternson-
rAAV-hSyn-GCaMp6m-WPRE-hGH polyAVirus construct--
pAAV.hSynap-FLEX.SF-iGluSnFR.A184VVirus constructPI: Looger. Activity: Cre dependent-
pAAV-Syn-ChR2(H134R)-GFPVirus constructActivity: Constitutive. PI: Boyden-
pAAV-FLEX-DTR-GFPVirus constructExpression System: CBA-driven, Cre-dependent. Activity: Cre-dependent expression of diphtheria toxin receptor fused to GFP for studying cell ablation.. PI: Jessell, Azim-
AAV9-hsyn-DIO-DA3.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
hDlx-HBB-chI-hERT2_Cre_hERT2-WPRE-SV40p(A)Virus constructsingle-stranded genome-
AAV-CaMKIIa-C1V1(E122T/E162T)-TS-mCherryVirus constructThird-generation Optogenetic Inhibition: eNpHR3.0-
hSyn1-GRAB(NE1h)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRubyVirus constructExpression System: Syn-driven, Cre-dependent. Activity: Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby for labeling of axon terminals.. PI: Luo-
CAG-dlox-tdTomato(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-TRE3G-CRE-WPRE-hGH polyAVirus construct--
Buzsaki32Silicon probe designNeuroNexus 32ch, 8 shanks, staggered, buzsaki-style, 200µm shank spacing (Buzsaki32)-
rAAV-TRE-tight-DIO-FLP-WPRE-hGHpAVirus construct--
AAV-EF1a-jGCaMP8s-WPREVirus constructPI: Looger. Activity: Constitutive-
AAV-CaMKIIa-eArchT3.0-EYFPVirus constructThird-generation Optogenetic Inhibition: Arch 3.0 & ArchT 3.0-
AAV-CaMKIIa-C1V1(t/t)-TS-p2A-mCherry-WPREVirus constructCell Filling Variants-
hEF1α-drox-hChR2(H134R)_EYFP(rev)-drox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
TRE3G-chI-eNpHR3.0_EYFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-CMV-NES-jRGECO1a-WPRE-hGH polyAVirus construct--
rAAV-Mecp2-CRE-WPRE-hGH polyAVirus construct--
mDlx-HBB-chI-dlox-mRuby3(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry-WPRE-hGH polyAVirus construct--
hEF1α-EGFP_2A-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV phSyn1(S)-DreO-bGHpAVirus constructPI: Zeng-
pAAV-CAG-FlpOVirus constructPI: Janelia-
pAV-EF1a-mRuby3-PGD2-1.0Virus constructTiter: ≥1*10E13vg/ml,50ul-
hSyn1-chI-dFRT-tdTomato(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV-CaMKIIa-hChR2(E123A)-EYFPVirus constructActivity: Constitutive. PI: Deisseroth-
AAV2-retro-hSyn-GCaMP6sVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
hSyn1-chI-dlox-iGluSnFR3.v857.PDGFR_2A_ChrimsonR_Kv2.1_3xHA(rev)-dlox-bGHp(A)Virus constructsingle-stranded genome-
pAAV-Ef1a-DIO-Synaptophysin-GCaMP6sVirus constructPI: Larsen. Activity: Cre dependent-
AAV9-EF1a-DIO-rACh1.7Virus constructTiter: ≥1*10E13vg/ml,50ul-
rAAV-VGLUT1-DIO-FLP-P2A-mCherry-WPRE-hGH polyAVirus construct--
hEF1α/hTLV1-dlox-mSyp1_tdTomato(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV-EF1a-BbTagBYVirus constructPI: Sanes. Activity: Cre dependent-
pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40Virus constructActivity: Cre dependent. PI: Harvey-
pAAV.Syn.GCaMP6m.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Constitutive-
hEF1α-dlox-hCofilin1_SuperNova(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV-nEF-Coff/Fon-iC++-EYFPVirus constructActivity: Flp dependent. PI: Deisseroth-
rAAV-nEF1α-EYFP-WPRE-hGH polyAVirus construct--
hEF1α/hTLV1-dFRT-mSyp1_tdTomato(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-CMV-DIO-EGFP-F2A-TVA-T2A-RVG-WPREsVirus construct--
pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFPVirus constructActivity: Cre dependent. PI: Deisseroth-
FG105LCA (Ø 105/125µm, 0.22 NA)Optic fiber designØ105 µm Core Glass Clad Silica Multimode Optical Fiber, 0.22 NA. Bare fiber. Multimode Fiber, 0.22 NA, Low OH, Ø105 µm Core, 400 - 2400 nm-
rAAV-CaMKIIa-His-BFP-2A-TVA-WPRE-hGH polyAVirus construct--
rAAV-hSyn-GRAB-SST1.0-WPRE-hGH polyAVirus construct--
pAAV-Ef1a-mCherryVirus constructPI: Deisseroth. Activity: Constitutive-
hSyn1-chI-dlox-EBFP2_2A_DreO(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pAAV.GfaABC1D.GluSnFr.SV40Virus constructPI: Khakh. Activity: Constitutive-
hSyn1-chI-dFRT-tdTomato_2A_iCre(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV9-hSyn-AVP1.5Virus constructTiter: ≥1*10E13vg/ml,50ul-
pWPI-IRES-Bla-Ak-TMPRSS2Virus constructExpresses human TMPRSS2. Blasticidin resistance. PI: Best.-
pENN.AAV.GFAP.iGluSnFr.WPRE.SV40Virus constructPI: Looger. Activity: Constitutive-
hGFAP-hHBbI/E-loxP-EGFP_mRpL10a-loxP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
c-Fos-synI-tTA-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-TRE-tight-DIO-FLP-WPRE-hGH polyAVirus construct--
rAAV-CAG-DIO-FLP-WPRE-hGH polyAVirus construct--
rAAV-CaMKIIa-hM3D(Gq)-mCherry-WPREsVirus construct--

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

  • Optic fiber designs: Thin, flexible fibers used for transmitting light signals, crucial for optical neural imaging and optogenetic manipulation of neurons.
  • Silicon probe designs: Microfabricated devices designed for recording or stimulating neural activity, allowing for high-resolution interfacing with neural tissue.
  • Virus constructs: Genetically engineered viruses used for delivering genes into neurons, enabling the study of gene function or the manipulation of neural activity.
  • Wire electrodes: Thin metal wires used for recording electrical signals from or delivering electrical stimulation to neurons, essential for electrophysiological studies.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.