Consumables

AAV9-GFAP-hM3D(Gq)-mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
rAAV-cfos-TTA-TRE3g-mCherry-WPRE-bGH polyAVirus construct--
rAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPRE-hGH polyAVirus construct--
pTight-hChR2(H134R)_EYFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV7-CAG-mCherryVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
AAV-CAG-FLEX-GFPVirus constructControl Vectors-
HB830ZOptic fiber designSingle Mode Polarizing Fiber (Zing™), 830 nm, Bow-Tie-
AAV-CaMKII-hChR2(T159C)-p2A-EYFP-WPREVirus constructCell Filling Variants-
Human gRNA pooled library in lentiCRISPRv2Virus constructHuman sgRNA library with SpCas9. 76,441 sgRNAs targeting 19,114 genes. Puromycin selection. PI: Doench, Root.-
rAAV-hSyn-CRE-P2A-EGFP-WPREsVirus construct--
rAAV-mOXTR-DIO-mCherry-WPRE-hGH polyAVirus construct--
hSyn1-dlox-GCaMP6m_mRpL10a(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-GfaABC1D-cATP1.0(chick)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV-CAG-hChR2-H134R-tdTomatoVirus constructActivity: Constitutive. *. PI: Svoboda-
AAV9-hSyn-cATP1.0-TESVirus constructTiter: ≥1*10E13vg/ml,50ul-
AAV pCAG-FLEX-EGFP-WPREVirus constructPI: Zeng. Activity: Cre dependent-
rAAV-Dlx5/6-DIO-EYFP-WPRE-hGH polyAVirus construct--
rAAV-TH-NLS-CRE-WPRE-hGH polyAVirus construct--
rAAV-nEf1α-FDIO-RVG-WPRE-hGH polyAVirus construct--
AAV9-hsyn-DIO-NE2hVirus constructTiter: ≥1*10E13vg/ml, 50ul-
hSyn1-lox71-ePdCO_mScarlet(rev)-lox66-WPRE-hGHp(A)Virus constructsingle-stranded genome-
A4x4-3mm-200-200-177Silicon probe designSpecial Order-
FG200LEAOptic fiber designØ200 µm Core Glass Clad Silica Multimode Optical Fiber, 0.22 NA-
AiE0780m-HBB-chI-iCre-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-EF1α-DIO-hM4D(Gi)-EYFP-WPREsVirus construct--
rAAV-hSyn-Chronos-EGFP-WPRE-bGH polyAVirus construct--
shortCAG-dlox-NES_jRCaMP1b(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hSyn1-dlox-mCherry(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-Jaws_KGC_tdTomato_ER2-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-CaMKIIa-hChR2(H134R)-EYFP-WPRE-hGH polyAVirus construct--
mCaMKIIα(short)-eOPN3_mScarlet-WPRE-synp(A)Virus constructsingle-stranded genome-
AAV5-GfaABC1D-Ado1.0m-mRuby3Virus constructTiter: ≥1*10E13vg/ml,50ul-
HN1550POptic fiber designSingle Mode Highly Nonlinear Fiber, +1 Anomalous Dispersion at 1550 nm-
AAV9-hSyn-GCaMP6m-P2A-nls-dTomatoVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
rAAV-D2-CRE-EGFP-WPRE-bGH polyAVirus construct--
pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3Virus constructActivity: Cre dependent. PI: Adesnik-
hCMV-chI-Lck_EGFP-SV40p(A)Virus constructself-complementary genome-
AAV9-hSyn-tdTomatoVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
rAAV-hSyn-NES-His-CaMPARI2-Flag-HA-Myc-WPRE-SV40 polyAVirus construct--
A8x4-tet-5mm-150-200-121Silicon probe designtetrode to tetrode distance: 150µm distance from first to last tetrode: 450µm See company catalog: 2018ProbeCatalog_20180515_Web, p.66-
rAAV-CaMKIIa-DIO-ChETA(E123A R134H)-EYFP-WPRE-hGH polyAVirus construct--
S5E2-HBB-chI[3x:sh(NS)]-Lck_GCaMP6f-SV40p(A)Virus constructsingle-stranded genome-
hEF1α-dFRT-(pro)taCasp3_2A_TEVp(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
FT200EMTOptic fiber designØ200 µm Core, 0.39 NA, Low OH TECS-Clad Multimode Fiber-
rAAV-CAG-FLEX-jGCaMP7b-WPRE-SV40 polyAVirus construct--
rAAV-CAG-hChR2(H134R)-EYFP-WPRE-hGH polyAVirus construct--
CAG-GRAB(DA2m)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
mCaMKIIα-RCaMP1.07-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV-CaMKII-hChR2(E123A)-EYFPVirus constructSecond-generation Ultrafast Optogenetic Control-
CAG-dFRT-G(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV-mDlx-NLS-mRuby2Virus constructPI: Gradinaru. Activity: Constitutive-
pAAV-flex-taCasp3-TEVpVirus constructExpression System: EF1a-driven, Cre-dependent. Activity: Cre-dependent, bicistronic expression of designer pro-taCasp3 and TEVp for studying cell ablation.. PI: Shah, Wells-
pGP-AAV-syn-jGCaMP7s-WPREVirus constructPI: Kim. Activity: Constitutive-
rAAV-hSyn-DIO-ChrimsonR-mCherry-WPRE-hGH polyAVirus construct--
FG600UEA (Ø 600/660µm, 0.22 NA)Optic fiber designØ600 µm Core Glass Clad Silica Multimode Optical Fiber, 0.22 NA-
mCaMKIIα-JEDI[1P]_rKv2.1-WPRE-hGHp(A)Virus constructsingle-stranded genome-
scAAV6-CMV-GFPVirus constructTiter: ≥5*10E12vg/ml Volume: 50ul-
hCD68-EGFP_iCre-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-EF1α-DIO-dLight1.2-WPRE-pAVirus construct--
hSyn1-chI-dlox-MTS_roGFP2_hGrx1(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-CMV-DIO-GCaMP6s-P2A-hChR2(H134R)-WPRE-hGH polyAVirus construct--
AAV2-hSyn-FlpVirus constructTiter: ≥5*10E12vg/ml. Volume: 50ul-
rAAV-hSyn-iGABASnFR-WPRE-SV40 polyAVirus construct--
hSyn1-chI-dlox-oCHIEF_tdTomato(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-EF1α-DIO-RVG-WPRE-hGH polyAVirus construct--
CAG-dFRT-hCHR2(H134R)_mCherry(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-EBFP2_2A_iCre-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pAAV-synapsin-HaloCaMP1a-EGFPVirus constructPI: Schreiter. Activity: Constitutive-
hSyn1-dlox-tdTomato(R415H)_2A_mSyp1_EGFP(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-Peredox_mCherry-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-dlox-mTagBFP2_2A_hM4D(Gi)_HA(2x)_rNrxn1(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
shortCAG-dlox-EGFP(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hSyn1-chI-pHRed-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV1-hSyn-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
hSyn1-dlox-hM3D(Gq)_mCherry(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV5-GfaABC1D-NE2hVirus constructTiter: ≥1*10E13vg/ml,50ul-
YB1200-4/125Optic fiber designYtterbium Doped Single Mode Fiber, 4.4 µm MFD-
AAV SYN PSAM4 GlyR IRES EGFPVirus constructCre requirement: none. PI: Sternson-
pAAV-EF1a-iFlpVVirus constructPI: Zeng-
mCaMKIIα-EGFP_mRpL10a-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-Ef1α-mCherry-WPRE-hGH polyAVirus construct--
hEF1α-H2B_EYFP-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pGP-AAV-CAG-FLEX-jGCaMP7s-WPREVirus constructPI: Kim. Activity: Cre dependent-
P-10/125DC-PMOptic fiber designPassive LMA PM Fiber, Matched to YB1200-10/125DC-PM. The supplier provides a numerical aperture of 0.08 - 0.085.-
A1x32-Poly2-5mm-50s-177Silicon probe designNeuroNexus 32ch, 1 shank, poly 2 (A1x32-Poly2-5mm-50s-177)-
rAAV-hSyn-Con Fon-hM4D(Gi)-EGFP-WPRE-hGH polyAVirus construct--
FP400URTOptic fiber design0.50 NA, Ø400 µm Core Multimode Fiber, High OH-
AAV-CAG-FLEX-Arch-GFPVirus constructNeural Silencers (no AAV6)-
hCMV-chI-mGreenLantern-SV40p(A)Virus constructself-complementary genome-
SM1950Optic fiber designSingle Mode Fiber, 1850 - 2200 nm, Ø125 µm Cladding-
RG-pLV-GAG-KanVirus constructFormat: Plasmid DNA, E. Coli Selection: Kan, Total Length: 8138 bp-
hEF1α-dFRT-iRFP670(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
H4Silicon probe designCambridge Neurotech H4, 32ch, 1 shanks, linear, 25µm spacing-
YB1200-25/250DCOptic fiber designYtterbium-Doped LMA Double-Clad Fiber, 25 µm Core Diameter-
hSyn1-chI-dlox-mTagBFP_2A_hM4D(Gi)_HA(2x)_rNrxn1(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
460HPOptic fiber designSingle Mode Fiber, 450 - 600 nm, Ø125 µm Cladding-
AAV2 retro-hSyn-Cre-P2A-GFPVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
hSyn1-dlox-mCBP_EGFP_2A_mSyp1_mRuby(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-hSyn-OXT1.7Virus constructTiter: ≥1*10E13vg/ml,50ul1
A4x8-8mm-100-500-703Silicon probe designSpecial Order-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

  • Optic fiber designs: Thin, flexible fibers used for transmitting light signals, crucial for optical neural imaging and optogenetic manipulation of neurons.
  • Silicon probe designs: Microfabricated devices designed for recording or stimulating neural activity, allowing for high-resolution interfacing with neural tissue.
  • Virus constructs: Genetically engineered viruses used for delivering genes into neurons, enabling the study of gene function or the manipulation of neural activity.
  • Wire electrodes: Thin metal wires used for recording electrical signals from or delivering electrical stimulation to neurons, essential for electrophysiological studies.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.