Consumables

pGP-AAV-syn-jGCaMP7f-WPREVirus constructPI: Kim. Activity: Constitutive-
AAV9-hSyn-eCB2.0-mScarletVirus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV9-hSyn-CCK2.3Virus constructTiter: ≥1*10E13vg/ml, 50ul-
hCD68-EGFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-CFOS-EYFP-WPRE-hGH polyAVirus construct--
pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2]Virus constructActivity: Cre dependent. PI: Boyden-
hEF1α-dlox-hChR2(H134R)_mCherry(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-ETV1-CRE-WPRE-hGH polyAVirus construct--
ER80-4/125-HD-PMOptic fiber designSingle Mode Erbium-Doped PM Fiber, 80.0 dB/m Absorption at 1530 nm, High Dispersion-
rAAV-Cfos720-CreERT2-WPRE-hGHpAVirus construct--
AAV1/2-CMV-CBA-Null/Empty-WPRE-BGH-polyAVirus constructTiter : >5x10^12 GC/mL-
rAAV-VGAT1-hM4D(Gi)-mCherry-WPRE-hGH polyAVirus construct--
rAAV-CBh-CRE-WPRE-bGH polyAVirus construct--
AAV9-hsyn-ATP1.0mut(B09 N283A)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
hGFAP-hHBbI/E-LiLac-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-CAG-CaMPARI2-WPRE-pAVirus construct--
hEF1α-dlox-mCherry-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
mCaMKIIα(short)-TVA950_mCherry_2A_G-WPRE3-synp(A)-SV40p(A)Virus constructsingle-stranded genome-
rAAV-RAM-d2TTA::TRE-NLS-mKate2-WPRE-pAVirus construct--
pTight-NLS_mKate2-WPRE-bGHp(A)-rp(A)-pRAM-d2tTA-SV40p(A)Virus constructsingle-stranded genome-
pENN.AAV.EF1a.eGFP.WPRE.rBGVirus constructPI: Wilson. Activity: Constitutive-
SHB1500Optic fiber designSingle Mode Spun Polarization-Maintaining Fiber, 1550 nm, Ø125 µm Cladding. The supplier provides a numerical aperture of 0.13 - 0.16.-
hU6-sgRNA(mβ-Actin)-HDR(EGFPmono_mβ-Actin)Virus constructsingle-stranded genome-
FP600ERTOptic fiber design0.50 NA, Ø600 µm Core Multimode Fiber, Low OH-
pAAV-hDlx-Flex-dTomato-Fishell_7Virus constructPI: Fishell. Activity: Cre dependent-
pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184SVirus constructPI: Looger. Activity: Cre dependent-
rAAV-EF1α-DIO-CaMPARI-WPRE-hGH polyAVirus construct--
AAV2-CAG-CreVirus constructTiter: ≥5*10E12vg/ml. Volume: 50ul-
rAAV-EF1α-DIO-CaMPARI2-WPRE-pAVirus construct--
AAV9-EF1a-DIO-hM3D(Gq)-mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
AAV-EF1a-mCherry-IRES-Cre-WPREVirus constructINTRSECT (AAV5)-
hSyn1-grpLight1.2-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pAAV-Ef1a-Con/Fon-GCaMP6FVirus constructPI: Deisseroth. Activity: Cre and Flp dependent-
hCMV-chI-loxP-TurboRFPdest1-loxP-SV40p(A)Virus constructself-complementary genome-
mMBP-mTagBFP2_iCre-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pTight-NLS_dTomato-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pENN.AAV.hSyn.Cre.hGHVirus constructPI: Wilson-
AAV9-hSyn-DIO-NE1m(NE2.1)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV.CamKII.GCaMP6s.WPRE.SV40Virus constructPI: Wilson. Activity: Constitutive-
mCaMKIIα(short)-iGECI_NES-WPRE-SV40p(A)Virus constructsingle-stranded genome-
AAV9-hSyn-DIO-hM4D(Gi)-mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
HN1550Optic fiber designSingle Mode Highly Nonlinear Fiber, -1 Normal Dispersion at 1550 nm-
hEF1α-dlox-LACTB_V5_dAPEX2(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-CAG-DIO-Arch-EYFP-WPRE-hGH polyAVirus construct--
hGFAP-chI-BioID2_BioID2_HA-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-TRE3g-mCherry-WPRE-hGH polyAVirus construct--
shortCAG-dlox-dTomato(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-TRE3g-hChR2(E123T/T159C)-mCherry-WPRE-hGH polyAVirus construct--
AAV9-EF1a-DIO-hChR2(H134R)-mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
PRS2(8x)-hDBH-chI-EYFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI[1x:sh(NS)]-EGFP-WPRE-SV40p(A)Virus constructsingle-stranded genome-
TRE-hM4D(Gi)_mCherry-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-EF1α-DIO-DA1m-WPRE-PAVirus construct--
AAV-CaMKIIa-OptoB2-EYFPVirus constructOpto-XRs  (AAV5)-
rAAV-Ef1α-DIO-mCherry-2A-TK-WPRE-hGH polyAVirus construct--
pAAV-hSyn-GRAB_NE1mVirus constructPI: Li. Activity: Constitutive-
rAAV-Cfos-D2-EGFP-WPRE-hGHpAVirus construct--
rAAV-hSyn-iGluSnFR(A184S)-WPRE-hGH polyAVirus construct--
AAV phSyn1(S)-FlpO-bGHpAVirus constructPI: Zeng-
hU6-sgRNA(mCry2-v1)-hU6-sgRNA(mCry2-v2)-hSyn1-mCherry_KASH-hGHp(A)Virus constructsingle-stranded genome-
SM980-5.8-125Optic fiber designSingle Mode Fiber, 980 - 1550 nm, Ø125 µm Cladding-
rAAV-hSyn-NE1hVirus construct--
hSyn1-chI-dlox-FLIP(c.-o.)(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
mCaMKIIα-hChR2(C128S/D156A)_EYFP-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-GRAB(DA2m)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-EF1α-DIO-TVA-EGFP-WPRE-hGH polyAVirus construct--
AAV-CaMKIIa-hChR2(C128S/D156A)-mCherry Virus constructStabilized Step Function Opsins: SSFOs-
AAV9-hsyn-OXT1.8Virus constructTiter: ≥1*10E13vg/ml,50ul-
hEF1α-dlox-myr_TagRFP-T_2A_(post)eGRASP(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-TRPV1-GCaMP6s-WPRE-bGH polyAVirus construct--
shortCAG-dlox-3xNLS_mScarlet3_2A_WGA2_FLPo(rev)-dlox-bGHp(A)Virus constructsingle-stranded genome-
AAV9-EF1a-DIO-hChR2(H134R)-EYFPVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
pAAV-hSyn Con/Fon EYFPVirus constructPI: Deisseroth. Activity: Cre and Flp dependent-
pAAV-S5E2-Gq-P2A-dTomatoVirus constructCre requirement: none. PI: Dimidschstein-
hGFAP-hHBbI/E-ATeam1.03YEMK-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-iRFP670-WPRE3-bGHp(A)Virus constructself-complementary genome-
PFD-rAAV-CaMKIIa-DA1h-WPRE-hGH polyAVirus construct--
pAAV-CaMKIIa-mCherryVirus constructPI: Deisseroth. Activity: Constitutive-
rAAV-EF1α-EGFP-P2A-CRE-WPRE-hGH polyAVirus construct--
pENN.AAV.CamKII 0.4.Cre.SV40Virus constructPI: Wilson-
pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-PuroVirus constructExpresses SARS-CoV-2 orf3a protein. Puromycin resistance. EF1a promoter. PI: Krogan.-
CaMKII-somBiPOLES-mCeruleanVirus constructActivity: Constitutive. PI: Wiegert-
rAAV-SST-CRE-bGH polyAVirus construct--
hSyn1-GRAB(NE2m)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
FG1000LEA (Ø 1000/1100µm, 0.22 NA)Optic fiber designØ1000 µm Core Glass Clad Silica Multimode Optical Fiber, 0.22 NA-
AAV9-hsyn-DIO-rDAmutVirus constructTiter: ≥1*10E13vg/ml,50ul-
rAAV-cfos-CRE-WPRE-hGH polyAVirus construct--
AAV9-EFla-DIO-C1V1-TS-mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
rAAV-D1(SP)-CRE-WPRE-bGH polyAVirus construct--
rAAV-GfaABC1D-hM3D(Gq)-mCherry-WPRE-SV40 polyAVirus construct--
EEG screwsSingle wire electrodeSmall EEG electrode which consists of a stainless steel jeweler’s screw welded to a stainless steel lead contact. Electrodes can be easily turned into holes that have been pre-drilled in the skull. The common screw 000-120 thread and ~0.8mm OD, with a length of 2-3mm.-
hSyn1-chI-EGFP_PA-Rac1-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonoptVirus constructPI: Podgorski. Activity: Cre dependent-
FG250LAOptic fiber designCoreless Termination Fiber, Ø250 µm Glass Rod-
pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPREVirus constructActivity: Constitutive. PI: Deisseroth-
AAV9-hsyn-DIO-AVP2.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
pAAV-hSynapsin1-axon-GCaMP6fVirus constructPI: Tian. Activity: Constitutive-
hEF1α-H2B_mKate2-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV1-hsyn-NE2m(NE3.1)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]Virus constructPI: Boyden. Activity: Cre dependent-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

  • Optic fiber designs: Thin, flexible fibers used for transmitting light signals, crucial for optical neural imaging and optogenetic manipulation of neurons.
  • Silicon probe designs: Microfabricated devices designed for recording or stimulating neural activity, allowing for high-resolution interfacing with neural tissue.
  • Virus constructs: Genetically engineered viruses used for delivering genes into neurons, enabling the study of gene function or the manipulation of neural activity.
  • Wire electrodes: Thin metal wires used for recording electrical signals from or delivering electrical stimulation to neurons, essential for electrophysiological studies.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.