Consumables

rAAV-CaMKIIa-hChR2(H134R)-mCherry-WPRE-hGH polyAVirus construct--
rAAV-D1-fDIO-CRE-mCherry-WPRE-bGH polyAVirus construct--
rAAV-CaMkIIa-Tau-tdtomato-WPREsVirus construct--
pAAV-hsyn-Jaws-KGC-GFP-ER2Virus constructActivity: Constitutive. PI: Boyden-
hSyn1-chI-GreenGlifon4000-WPRE-bGHp(A)Virus constructsingle-stranded genome-
S5E2-HBB-chI-dTomato_2A_NLS_dTomato-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hSyn1-scFLARE2-bGHp(A)Virus constructsingle-stranded genome-
AAV1-EFS-DIO-TAC01Virus constructTiter: ≥1*10E13vg/ml,50ul-
AAV-Ef1a-DIO eNpHR 3.0-EYFPVirus constructActivity: Cre dependent. PI: Deisseroth-
AAV8-U6-shRNA(scramble)-CMV-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
mCaMKIIα-GCaMP6m_2A_ChRmine_MTS_Kv2.1-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV5-GfaABC1D-rATP0.5Virus constructTiter: ≥1*10E13vg/ml,50ul-
rAAV-hSyn-axon-GCaMP6f-WPRE-hGH polyAVirus construct--
PFD-rAAV-EF1a-fDIO-iGABASnFR-WPRE-hGH polyAVirus construct--
hSyn1-eOPN3_mScarlet-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-CMV-Mito-dsRed-WPRE-bGH polyAVirus construct--
FFH10026Optic fiber designØ100 µm Core, 0.26 NA, Double-Clad InF₃ Multimode Fiber-
rAAV-CAG-hChR2(H134R)-mCherry-nrxn1a rev-WPRE-hGH polyAVirus construct--
hSyn1-chI-IRES_mCitrine-WPRE-bGHp(A)Virus constructsingle-stranded genome-
shortCAG-dlox-hM4D(Gi)_mCherry(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
A1x32-7mm-100-1250Silicon probe designSpecial Order-
rAAV-RAM-d2TTA::TRE-ArchT-EGFP-WPRE-pAVirus construct--
AAV9-hSyn-OXT1.5Virus constructTiter: ≥1*10E13vg/ml,50ul-
GF3Optic fiber designPhotosensitive SM Fiber, 1500 - 1600 nm, 7.5 µm MFD, 0.16 NA-
pAAV.Syn.NES.jRCaMP1a.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Constitutive-
AAV-CAG-ArchT-GFPVirus constructNeural Silencers-
hEF1α-dFRT-EGFP_2A_FLAG_TeTxLC(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-hSyn_ATP1.1mVirus constructTiter: ≥1*10E13vg/ml,50ul-
AAV9-hSyn-gACh4mVirus constructTiter: ≥1*10E13vg/ml,50ul-
hSyn1-FLIP(c.-o.)-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-TBG-hChR2-EGFP-WPRE-hGH polyAVirus construct--
hSyn1-ConVERGD(H2BJ_3xHA_2A_G (CVS-N2c))-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-CMV-DIO-EGFP-WPRE-hGH polyAVirus construct--
rAAV-CAG-DIO-EGFP-WPRE-hGH polyAVirus construct--
AAV8-ALB-CreVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
pAAV-hSyn-JEDI-2P-WPREVirus constructPI: St-Pierre. Activity: Constitutive-
hEF1α-lox2272mut-mCherry-loxPmut-DTA(rev)-dloxmut-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-Ki67-hChR2-EYFP-WPRE-hGH polyAVirus construct--
hGFAP-hHBbI/E-dlox-dTomato-EGFP(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
FT1000UMTOptic fiber designØ1000 µm Core, 0.39 NA, High OH TECS-Clad Multimode Fiber-
AAV5-GfaABC1D-ATP1.1mVirus constructTiter: ≥1*10E13vg/ml,50ul-
pAAV.CAG.Flex.GCaMP6m.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Cre dependent-
pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpAVirus constructCre requirement: Flp-dependent. PI: Gether-
hSyn1-ATeam1.03-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV-CaMKII-hChR2(T159C)-p2A-mCherry-WPREVirus constructCell Filling Variants-
pZac2.1 gfaABC1D-lck-GCaMP6fVirus constructPI: Khakh. Activity: Constitutive-
hSyn1-chI-dFRT-eArchT3.0_EYFP-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-GfaABC1D-hM3D(Gq)-mCherry-SV40 polyAVirus construct--
pAAV-EF1a-N-CretrcintGVirus constructPI: Cepko-
rAAV-EF1α-DIO-GRAB-NEmut-WPRE-hGH polyAVirus construct--
pAAV.Syn.NES-jRGECO1a.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Constitutive-
AAV9-hsyn-VIP1.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV9-hsyn-AVP2.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
rAAV-PTH-CRE-WPRE-hGH polyAVirus construct--
AAV1-EFS-DIO-TAC0.1Virus constructTiter: ≥1*10E13vg/ml, 50ul-
hSyn1-chI-dlox-EGFP(rev)-dlox-SV40p(A)Virus constructself-complementary genome-
AAV9-hsyn-Ado1.0med(Ado1.0-M270H)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV-CAG-Arch-GFPVirus constructNeural Silencers (no AAV6)-
P-6/125DCOptic fiber designPassive Single Mode Double-Clad Fiber, 7 µm Core, Matched to YB1200-6/125DC-
hSyn1-chI-dlox-MaLionR(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-EF1α-DIO-hChR2(E123T/T159C)-EYFP-WPRE-hGH polyAVirus construct--
pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-PuroVirus constructExpresses SARS-CoV-2 N protein. Puromycin resistance. EF1a promoter. PI: Krogan.-
hSyn1-Aion_ts_Citrine_ER-WPRE-bGHp(A)Virus constructsingle-stranded genome-
GIF625-100Optic fiber design100 m Spool of Ø62.5 µm Core Graded-Index Multimode Optical Fiber-
AAV-CaMKIIa-eArch3.0-EYFPVirus constructThird-generation Optogenetic Inhibition: Arch 3.0 & ArchT 3.0-
CAG-dFRT-rSyp1_EGFP(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV9-hSyn-OXT1.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
H3Silicon probe designCambridge Neurotech H3, 64ch, 1 shank, linear, 20µm spacing-
SM1500G80Optic fiber designSingle Mode Fiber, 1520 - 1650 nm, Ø80 µm Cladding-
mCaMKIIα-mTagBFP2-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV.CAG.GCaMP6f.WPRE.SV40Virus constructPI: Kim , GENIE. Activity: Constitutive-
AAV5-hSyn-DIO-GCaMP6s-P2A-nls-dTomatoVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
rAAV-hSyn-CRE-EGFP-WPRE-hGH polyAVirus construct--
A2x2-tet-3mm-150-150-121Silicon probe designtetrode to tetrode distance: 150µm See company catalog 2018_ProbeCatalog_20180515_Web, p.41-
hCMV-dlox-SaCas9(rev)-dlox-bGHp(A)-hU6-sgRNA(mTh)Virus constructsingle-stranded genome-
AAV9-CAG-cyto-Ado-mutVirus constructTiter: ≥1*10E13vg/ml,50ul-
PFD-rAAV-hSyn-ASAP3-WPRE-hGH polyAVirus construct--
AAV9-hSyn-AC-UK1.0Virus constructTiter: ≥1*10E13vg/ml,50ul-
hSyn1-chI-dlox-paEGFP_rOmp25(rev)-dlox-SV40p(A)Virus constructself-complementary genome-
rAAV-hSyn-CCK2.3Virus construct--
shortCAG-dFRT-(post)mGRASP_2A_mScarlet-I(rev)-dFRT-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hSyn1-chI-dlox-EBFP2_2A_DreO-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
hEF1α-dlox-EGFP-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-foxb1-CRE-WPRE-hGH polyAVirus construct--
pAAV-Ef1a-DIO C1V1 (t/t)-TS-EYFPVirus constructActivity: Cre dependent. PI: Deisseroth-
rAAV-mDLx-hChR2(H134R)-mCherry-WPRE-hGH polyAVirus construct--
S5E2-HBB-chI-GCaMP6f-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rAAV-cfos-D2mCherry-WPRE-hGH polyAVirus construct--
PFD-rAAV-cTNT-DA1h-WPREsVirus construct--
rAAV-CRH-DIO-hM3D(Gq)-EGFP-WPREsVirus construct--
hGFAP-EBFP2-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV.CMV.ffLuciferase.SV40Virus constructPI: Wilson. Activity: Constitutive-
FG600LEAOptic fiber designØ600 µm Core Glass Clad Silica Multimode Optical Fiber, 0.22 NA-
pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3Virus constructPI: Tian. Activity: Constitutive-
hDlx-HBB-chI-EGFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV9-hSyn-TAC1.0Virus constructTiter: ≥1*10E13vg/ml,50ul-
FG200UEP Optic fiber designØ200 µm Core Polyimide-Coated Multimode Fibers-
pAAV-CAG-DIO-EYFPVirus constructPI: Gradinaru. Activity: Cre dependent-
hU6-sgRNA(mPer1-v3)-hU6-sgRNA(mPer2-v1)-hU6-sgRNA(mPer2-v2)-hSyn1-mCherry_KASH-hGHp(A)Virus constructsingle-stranded genome-
hEF1α-loxFAS-hChR2(H134R)_mCherry-loxFAS-WPRE-hGHp(A)Virus constructsingle-stranded genome-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

  • Optic fiber designs: Thin, flexible fibers used for transmitting light signals, crucial for optical neural imaging and optogenetic manipulation of neurons.
  • Silicon probe designs: Microfabricated devices designed for recording or stimulating neural activity, allowing for high-resolution interfacing with neural tissue.
  • Virus constructs: Genetically engineered viruses used for delivering genes into neurons, enabling the study of gene function or the manipulation of neural activity.
  • Wire electrodes: Thin metal wires used for recording electrical signals from or delivering electrical stimulation to neurons, essential for electrophysiological studies.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.