Consumables

hGFAP-CapChR2_2A_3xNLS_mScarlet3-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV-CaMKIIa-SwiChRCA-TS-EYFPVirus constructSwiChRCA  (AAV2, 5, DJ)-
PFD-rAAV-EF1α-DIO-GRAB_eCB2.0-WPREsVirus construct--
AAV-EF1a-DIO-C1V1(E122T/E162T)-TS-mCherryVirus constructThird-generation Optogenetic Inhibition: eNpHR3.0-
AAV-EF1a-DIO-OptoD1-mCherryVirus constructOpto-XRs  (AAV5)-
rAAV-Dlx5-6-DIO-hM3(Gq)-mCherry-WPRE-hGH polyAVirus construct--
hU6-sgRNA(mCry1-v2)-hU6-sgRNA(mCry2-v1)-hU6-sgRNA(mCry2-v2)-hSyn1-mCherry_KASH-hGHp(A)Virus constructsingle-stranded genome-
FG273UECOptic fiber designØ273 µm Core, 0.22 NA, High OH TECS Double-Clad Multimode Fiber-
hSyn1-chI-dFRT-CoChR_EGFP(rev)-dFRT-WPRE-bGHp(A)Virus constructsingle-stranded genome-
Neuropixels 2.0Silicon probe design-2
hSyn1-chI-lox71-GCaMP6m_mRpL10a-lox66-WPRE-bGHp(A)Virus constructsingle-stranded genome-
scAAV2/5-CBh–Empty/Null–WPRE3-enSV40pAVirus constructTiter : >5x10^12 GC/mL-
hCD68-mCherry-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40Virus constructPI: Schreiter. Activity: Constitutive-
rAAV-cTNT-iCRE-IRES2-tdTamato-polyAVirus construct--
TRE3G-chI-TVA950_2A_mTagBFP2_3xNLS_2A_G (CVS-N2c)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV.hSyn-FLEX.iGABASnFR.F102GVirus constructPI: Looger. Activity: Cre dependent-
Buzsaki256Silicon probe designNeuroNexus 256ch, 8 shank, linear 50µm vertical pitch, 300µm shank spacing (Buzsaki256)-
pAAV-hSyn-eNpHR 3.0-EYFPVirus constructActivity: Constitutive. PI: Deisseroth-
rAAV-TRE3G-hChR2(H134R)-mCherry-WPRE-hGHpAVirus construct--
pAAV-CaMKIIa-hChR2(E123A)-mCherryVirus constructActivity: Constitutive. PI: Deisseroth-
AAV9-GFaABC1D-eCBmutVirus constructTiter: ≥1*10E13vg/ml,50ul-
rAAV-mDlx-DIO-hM4D(Gi)-mCherry-WPRE-hGH polyAVirus construct--
pAAV_hSyn-PdCO-EGFP-WPREVirus constructActivity: Constitutive. PI: Yizhar-
AAV9-hsyn-cATP1.0(chick)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
hDlx-HBB-chI-dlox-Jaws_KGC_tdTomato_ER2(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-dlox-DTA(rev)-dlox-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hEF1α-dlox-hM3D(Gq)_mCherry(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV-hsyn-GRAB_rDA1hVirus constructPI: Li. Activity: Constitutive-
FZM60020Optic fiber designØ600 µm Core, 0.20 NA, ZBLAN Multimode Fiber-
hPDGFβ-SV40I-DsRed2Mono_2A_Cre-WPRE-SV40p(A)Virus constructsingle-stranded genome-
P-25/250DCOptic fiber designPassive LMA Double-Clad Fiber, 25 µm Core, Matched to YB1200-25/250DC-
AAV9-EF1a-DIO-mCherryVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
rAAV-CMV-EGFP-3XFlag-WPRE-hGH polyAVirus construct--
AAV-hSyn-mCherryVirus constructControl Fluorophores-
rAAV-Ef1α-DIO-His-BFP-2A-TVA-WPRE-hGH polyAVirus construct--
HB1550ZOptic fiber designSingle Mode Polarizing Fiber (Zing™), 1550 nm, Bow-Tie-
rAAV-CAG-mCherry-T2A-hM4D-nrxn1a rev-WPRE-SV40 polyAVirus construct--
rAAV-mDlx-DIO-hChR2(H134R)-mCherry-WPRE-hGH polyAVirus construct--
pGP-AAV-CAG-FLEX-jGCaMP8s-WPREVirus constructPI: GENIE. Activity: Cre dependent-
AAV-EF1a-DIO-hChR2(H134R)-mCherryVirus constructCre-inducible AAV: DIO-AAV-
AAV9-GfaABC1D-Ado1.0medVirus constructTiter: ≥1*10E13vg/ml, 50ul-
AAV9-GfaABC1D-ATP1.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
scAAV7m8-CMV-GFPVirus constructTiter: ≥5*10E12vg/ml Volume: 50ul-
AAV9-hsyn-MT2.0Virus constructTiter: ≥1*10E13vg/ml, 50ul-
hCMV-chI-GreenGlifon4000-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-TBG-Luciferase-WPRE-bGH polyAVirus construct--
AAV9-hSyn-DIO-EGFP-CAAXVirus constructTiter: ≥1*10E13vg/ml,50ul-
rAAV-GAD67-CRE-mCherry-WPRE-hGH polyAVirus construct--
pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPREVirus constructPI: Deisseroth. Activity: Constitutive-
hSyn1-chI-cdATeam1.03-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV-hSyn-ChR2(E123A)-EYFPVirus constructSecond-generation Ultrafast Optogenetic Control-
hSyn1-CaMPARI2(F391W/L398V)_h-alpha-tubulin-WPRE-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-chI-LiLac-WPRE-bGHp(A)Virus constructsingle-stranded genome-
pAAV.hSyn.CaMPARI.WPRE.SV40Virus constructPI: Looger. Activity: Constitutive-
rAAV-nEF1a-fDIO-hM3D(Gq)-EGFP-WPRE-hGH polyAVirus construct--
hSyn1-chI-H2BJ_mEGFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hGFAP-hChR2(H134R)_EYFP-WPRE-hGHp(A)Virus constructsingle-stranded genome-
pAAV-hSyn-DIO-hM3D(Gq)-mCherryVirus constructCre requirement: Cre-dependent. PI: Roth-
hSyn1-chI-eArch3.0_EYFP-WPRE-bGHp(A)Virus constructsingle-stranded genome-
hSyn1-EGFP-SV40p(A)Virus constructself-complementary genome-
CAG-dFRT-GAP43_jGCaMP8m(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
AAV9-hSyn-DIO-hM3D(Gq)-mCherryVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
hEF1α/hTLV1-dFRT-RCaMP1.07(rev)-dFRT-WPRE-hGHp(A)Virus constructsingle-stranded genome-
rAAV-hSyn-hChR2(E123T/T159C)-EYFP-WPRE-hGH polyAVirus construct--
rAAV-Dlx5/6-EYFP-WPRE-hGH polyAVirus construct--
hGFAP-chI-MTS_roGFP2_hGRX1-4x6(miRT)-WPRE-bGHp(A)Virus constructsingle-stranded genome-
rAAV-nEF1α-fDIO-GCaMp6f-WPRE-hGH polyAVirus construct--
pAAV-CAG-RdLight1Virus constructPI: Tian. Activity: Constitutive-
AAV-EF1a-DIO-ChR2(E123A)-mCherryVirus constructSecond-generation Ultrafast Optogenetic Control-
AAV1-CMV-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
hSyn1-chI-nLightG-WPRE-bGHp(A)Virus constructsingle-stranded genome-
E2-64chSilicon probe design<p>Cambridge Neurotech E-series, 64ch, 4 shanks, edge, 250µm shank spacing</p>-
AAV-hSyn1-GCaMP6s-P2A-nls-dTomatoVirus constructPI: Ting. Activity: Constitutive-
rAAV-GFAP-eNpHR3.0-EYFP-WPREsVirus construct--
FG100LEPOptic fiber designØ100 µm Core, 0.22 NA, Low OH Polyimide-Coated Step-Index Multimode Fiber-
AAV pmSyn1-EBFP-CreVirus constructPI: Zeng-
AAV9-EFS-DIO-rNE1.1(rcNE1.1)Virus constructTiter: ≥1*10E13vg/ml, 50ul-
UM22-300 (Ø 300/330µm, 0.22 NA)Optic fiber designØ300 µm Core Solarization-Resistant, Polyimide-Coated Multimode Fiber-
AAV-EF1a-fDIO-EYFP-WPREVirus constructINTRSECT (AAV5 & DJ)-
AAV9-c-fos-GFPVirus constructTiter: ≥1*10E13vg/ml Volume: 50ul-
rAAV-CAG-mCherry-nrxn1a rev-WPRE-hGH polyAVirus construct--
hSyn1-dlox-ReaChR_mCitrine(rev)-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pAAV-Ef1a-Coff/Fon-oScarletVirus constructPI: Deisseroth. Activity: Flp dependent-
hEF1α-dlox-Igk_dAPEX2_KDEL(rev)-dlox-WPRE-hGHp(A)Virus constructsingle-stranded genome-
Mouse sgRNA library Brie in lentiGuide-PuroVirus constructMouse sgRNA library targeting 19,674 genes. 78,637 sgRNAs + 1000 controls. Puromycin selection. PI: Doench, Root.-
hSyn1-chI-Voltron2-ST-WPRE-bGHp(A)Virus constructsingle-stranded genome-
AAV CAMKII PSAM4 GlyR IRES EGFPVirus constructCre requirement: none. PI: Sternson-
mTH-EGFP-WPRE-SV40p(A)Virus constructsingle-stranded genome-
rTH-hChR2(H134R)_mRuby3-hGHp(A)Virus constructsingle-stranded genome-
hSyn1-hM4D(Gi)_mCherry-WPRE-hGHp(A)Virus constructsingle-stranded genome-
mCaMKIIα-hM4D(Gi)_mCherry-WPRE-hGHp(A)Virus constructsingle-stranded genome-
shortCAG-dlox-mCherry_2A_hM4D(Gi)_HA(2x)_rNrxn1-dlox-WPRE-SV40p(A)Virus constructsingle-stranded genome-
pAAV.GFAP.SF-iGluSnFR.A184SVirus constructPI: Looger. Activity: Constitutive-
AAV8-EF1a-DIO-GCaMP6s-P2A-nls-dTomatoVirus constructTiter: ≥1*10E13vg/ml. Volume: 50ul-
P-20/125DC-PMOptic fiber designPassive LMA PM Fiber, Matched to YB800-20/125DC-PM-
AAV-hSyn-SwiChRCA-TS-EYFPVirus constructSwiChRCA  (AAV2, 5, DJ)-
rAAV-GFAP-mcherry-WPRE-hGH polyAVirus construct--
rAAV-CAG-DIO-hChR2(H134R)-EYFP-WPREsVirus construct--
pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pAVirus constructPI: Bonhoeffer , Huebener , Rose. Activity: Cre dependent-

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Consumables

Consumables refer to items that are intended to be used once or have a limited lifespan before they need to be replaced. These include materials and products that are consumed or depleted during the course of experiments or procedures. Consumables are critical for maintaining the integrity, accuracy, and reproducibility of scientific experiments. In BrainSTEM, consumables are a shared model across users.

Types of consumable

  • Optic fiber designs: Thin, flexible fibers used for transmitting light signals, crucial for optical neural imaging and optogenetic manipulation of neurons.
  • Silicon probe designs: Microfabricated devices designed for recording or stimulating neural activity, allowing for high-resolution interfacing with neural tissue.
  • Virus constructs: Genetically engineered viruses used for delivering genes into neurons, enabling the study of gene function or the manipulation of neural activity.
  • Wire electrodes: Thin metal wires used for recording electrical signals from or delivering electrical stimulation to neurons, essential for electrophysiological studies.

Submission process

Anyone can submit consumables or submit changes to existing ones, but all submissions must be approved before they are available for usage. Please see existing entries for examples as to what to submit.

Fields

  • Name: The name of the consumable (required; must be unique).
  • Type: The type of consumable - see available types above (required).
  • Supplier: The supplier of the consumable (required).
  • Description: A description of the consumable.
  • Type specific fields: Each type has custom fields (required).

Permissions

Once a entry has been approved it becomes available to everyone.

API Access

The API allows for programmable access, enabling you to read, edit, and delete entries through the API. For details about the fields and data structure, refer to the documentation.